Abstract
The mechanism of CNBr activation of polysaccharide resins like Sepharose and Sephadex has been elucidated using recently published analytical procedures for the determination of cyanate esters and imido carbonates. It was found that on agarose-based resins coupling of ligand occurs predominantly via cyanate esters, and not via imidocarbonates as in the case of Sephadex. This explains the different behaviours of Sepharose and Sephadex during CNBr activation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 161-163 |
| Number of pages | 3 |
| Journal | Enzyme and Microbial Technology |
| Volume | 4 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 1982 |
| Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology
Keywords
- Polysaccharide resins
- activation mechanism
- cyanate ester
- cyanogen bromide
- imidocarbonate