Mechanism of activation of Sepharose and Sephadex by cyanogen bromide

Joachim Kohn, Meir Wilchek

Research output: Contribution to journalArticlepeer-review

61 Scopus citations


The mechanism of CNBr activation of polysaccharide resins like Sepharose and Sephadex has been elucidated using recently published analytical procedures for the determination of cyanate esters and imido carbonates. It was found that on agarose-based resins coupling of ligand occurs predominantly via cyanate esters, and not via imidocarbonates as in the case of Sephadex. This explains the different behaviours of Sepharose and Sephadex during CNBr activation.

Original languageEnglish (US)
Pages (from-to)161-163
Number of pages3
JournalEnzyme and Microbial Technology
Issue number3
StatePublished - May 1982
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology


  • Polysaccharide resins
  • activation mechanism
  • cyanate ester
  • cyanogen bromide
  • imidocarbonate


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