Mechanism of o-phenanthroline mediated inhibition of E. coli DNA polymerase I: Formation of template-primer-metal-phenanthroline complexes with resultant loss of catalytic activity

Kakkudiyil I. Abraham, Mukund Modak

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Inhibition of E. coli DNA polymerase I activity by 1,10 phenanthroline in the absence of reducing agents requires a high concentration of inhibitor (1-10 mM) depending upon the template primer used to direct the synthesis. We find that o-phenanthroline, unlike its non-chelating analogue, forms a divalent cation mediated complex with template-primers. Enzyme bound to such complexes is unable to catalyse either polymerization or nuclease functions.

Original languageEnglish (US)
Pages (from-to)567-576
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume115
Issue number2
DOIs
StatePublished - Sep 15 1983
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

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