Inhibition of E. coli DNA polymerase I activity by 1,10 phenanthroline in the absence of reducing agents requires a high concentration of inhibitor (1-10 mM) depending upon the template primer used to direct the synthesis. We find that o-phenanthroline, unlike its non-chelating analogue, forms a divalent cation mediated complex with template-primers. Enzyme bound to such complexes is unable to catalyse either polymerization or nuclease functions.
|Original language||English (US)|
|Number of pages||10|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Sep 15 1983|
All Science Journal Classification (ASJC) codes
- Molecular Biology