Mediating Ribosomal Competition by Splitting Pools

Jared Miller, M. Ali Al-Radhawi, Eduardo D. Sontag

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Synthetic biology constructs often rely upon the introduction of 'circuit' genes into host cells, in order to express novel proteins and thus endow the host with a desired behavior. The expression of these new genes 'consumes' existing resources in the cell, such as ATP, RNA polymerase, amino acids, and ribosomes. Ribosomal competition among strands of mRNA may be described by a system of nonlinear ODEs called the Ribosomal Flow Model (RFM). The competition for resources between host and circuit genes can be ameliorated by splitting the ribosome pool by use of orthogonal ribosomes, where the circuit genes are exclusively translated by mutated ribosomes. In this work, the RFM system is extended to include orthogonal ribosome competition. This Orthogonal Ribosomal Flow Model (ORFM) is proven to be stable through the use of Robust Lyapunov Functions. The optimization problem of maximizing the weighted protein translation rate by adjusting allocation of ribosomal species is formulated.

Original languageEnglish (US)
Title of host publication2021 American Control Conference, ACC 2021
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1897-1902
Number of pages6
ISBN (Electronic)9781665441971
DOIs
StatePublished - May 25 2021
Externally publishedYes
Event2021 American Control Conference, ACC 2021 - Virtual, New Orleans, United States
Duration: May 25 2021May 28 2021

Publication series

NameProceedings of the American Control Conference
Volume2021-May
ISSN (Print)0743-1619

Conference

Conference2021 American Control Conference, ACC 2021
Country/TerritoryUnited States
CityVirtual, New Orleans
Period5/25/215/28/21

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

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