Membrane-associated phosphatidylinositol kinase (ATP:phosphatidylinositol 4-phosphotransferase, EC 22.214.171.124) was partially purified 93-fold from Saccharomyces cerevisiae. Activity was dependent on magnesium ions (10 mM) and the optimum pH was 8.5. The apparent K(m) values for ATP and phosphatidylinositol were 0.21 mM and 71 μM, respectively. Activity was stimulated by sodium cholate and inhibited by sodium, potassium, lithium, and fluoride ions.
|Original language||English (US)|
|Number of pages||3|
|Journal||Journal of bacteriology|
|State||Published - Jan 1 1983|
All Science Journal Classification (ASJC) codes
- Molecular Biology