Membrane Biogenesis in the Presence of Ethanol

Amalia Slomiany, Maria Grabska, Ewa Grzelinska, Ken‐ichiro ‐i Yamaki, Chinnaswamy Kasinathan, Beatrix A. Slomiany, Bronislaw L. Slomiany

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

To investigate the effect of ethanol on the intracellular transport in gastric epithelial cells, the in vitro system, generating transport vesicles which transfer mucus glycoprotein apopeptide (apomucin) from rough endoplasmic reticulum (RER) to Golgi, was assembled. The vesicles, generated from gastric mucous cell RER microsomes and labeled with [3H]palmitic acid, were isolated from the maternal RER and characterized. The electron microscopy revealed that these RER products consisted of 80 to 100 nm smooth membrane vesicles, while the immunochemical analyses showed that they contain apomucin but were devoid of the characteristic integral proteins of the RER membrane. Incubation of apomucin transporting vesicles with Golgi in the presence of UDP‐[3H]galactose resulted in the formation of glycosylated mucin and fusion of the vesicles with Golgi. Formation of ER transport vesicles was dependent on the supply of lipid precursors, and the activity of phosphoglyceride and sphingolipid synthesizing enzymes. In the presence of 60 and 120 MM ethanol, the vesicles were formed, but their lipid composition was modified. The results suggest that ethanol‐induced membrane alterations are initiated at the early stages of the membrane biogenesis and are first reflected in the lipid composition of the intracellular transport vesicles.

Original languageEnglish (US)
Pages (from-to)1152-1161
Number of pages10
JournalAlcoholism: Clinical and Experimental Research
Volume16
Issue number6
DOIs
StatePublished - Dec 1992

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Toxicology
  • Psychiatry and Mental health

Keywords

  • Alcohol
  • Glycoprotein Transport
  • Intracellular Transport
  • Membrane Biogenesis
  • Phospholipid Synthesis
  • Transport Vesicles

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