Net lipid synthesis stops when glycerol is removed from a glycerol auxotroph of Bacillus subtilis. Under these conditions, the incorporation of proteins into cell membranes continues. The amount of protein in the membrane fraction and the total activity of succinic dehydrogenase both increase in the membrane fraction of the deprived cells. Membranes isolated from cells deprived of glycerol for one hour have approximately 12% lipid as compared to 21% lipid in normal cells. The membranes of deprived cells exhibit a greater buoyant density (1.19 g/ml.) than those from normal cells (1.17 g/ml.). When protein synthesis is inhibited by the growth of cells in the presence of chloramphenicol, isolated membranes have a 15 to 30% higher lipid content than that of normal cells. These findings are discussed in relation to the regulation of membrane composition.
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology