Membranes of Rhodopseudomonas sphaeroides. VI. Isolation of a fraction enriched in newly synthesized bacteriochlorophyll a-protein complexes

Robert Niederman, David E. Mallon, Lawrence C. Parks

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Radioactivity eventually destined for the chromatophore membrane of Rhodopseudomonas sphaeroides was shown in pulse-chase studies to appear first in a distinct pigmented fraction. This material formed an upper pigmented band which sedimented more slowly than chromatophores when cell-free extracts were subjected directly to rate-zone sedimentation on sucrose density gradients. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the purified fraction contained polypeptide bands of the same mobility as light-harvesting bacteriochlorophyll a and reaction center-associated protein components of chromatophores; these were superimposed upon cytoplasmic membrane polypeptides. The pulse-chase relation was confined mainly to the polypeptide components of these pigment-protein complexes. It is suggested that the isolated fraction may be derived from sites at which new membrane invagination is initiated.

Original languageEnglish (US)
Pages (from-to)210-220
Number of pages11
JournalBBA - Biomembranes
Volume555
Issue number2
DOIs
StatePublished - Aug 7 1979

Fingerprint

Chromatophores
Bacteriochlorophylls
Rhodobacter sphaeroides
Membranes
Peptides
Proteins
Radioactivity
Cell Extracts
Electrophoresis
Sedimentation
Pigments
Sodium Dodecyl Sulfate
Sucrose
Polyacrylamide Gel Electrophoresis
Cell Membrane
Light

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology

Keywords

  • (Rps. sphaeroides)
  • Bacteriochlorophyll-protein complex
  • Chromatophore
  • Membrane synthesis

Cite this

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Membranes of Rhodopseudomonas sphaeroides. VI. Isolation of a fraction enriched in newly synthesized bacteriochlorophyll a-protein complexes. / Niederman, Robert; Mallon, David E.; Parks, Lawrence C.

In: BBA - Biomembranes, Vol. 555, No. 2, 07.08.1979, p. 210-220.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Membranes of Rhodopseudomonas sphaeroides. VI. Isolation of a fraction enriched in newly synthesized bacteriochlorophyll a-protein complexes

AU - Niederman, Robert

AU - Mallon, David E.

AU - Parks, Lawrence C.

PY - 1979/8/7

Y1 - 1979/8/7

N2 - Radioactivity eventually destined for the chromatophore membrane of Rhodopseudomonas sphaeroides was shown in pulse-chase studies to appear first in a distinct pigmented fraction. This material formed an upper pigmented band which sedimented more slowly than chromatophores when cell-free extracts were subjected directly to rate-zone sedimentation on sucrose density gradients. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the purified fraction contained polypeptide bands of the same mobility as light-harvesting bacteriochlorophyll a and reaction center-associated protein components of chromatophores; these were superimposed upon cytoplasmic membrane polypeptides. The pulse-chase relation was confined mainly to the polypeptide components of these pigment-protein complexes. It is suggested that the isolated fraction may be derived from sites at which new membrane invagination is initiated.

AB - Radioactivity eventually destined for the chromatophore membrane of Rhodopseudomonas sphaeroides was shown in pulse-chase studies to appear first in a distinct pigmented fraction. This material formed an upper pigmented band which sedimented more slowly than chromatophores when cell-free extracts were subjected directly to rate-zone sedimentation on sucrose density gradients. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the purified fraction contained polypeptide bands of the same mobility as light-harvesting bacteriochlorophyll a and reaction center-associated protein components of chromatophores; these were superimposed upon cytoplasmic membrane polypeptides. The pulse-chase relation was confined mainly to the polypeptide components of these pigment-protein complexes. It is suggested that the isolated fraction may be derived from sites at which new membrane invagination is initiated.

KW - (Rps. sphaeroides)

KW - Bacteriochlorophyll-protein complex

KW - Chromatophore

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