Mercury resistance and bioremediation mediated by endophytic fungi

William Pietro-Souza, Felipe de Campos Pereira, Ivani Souza Mello, Fernando Ferrari Frutuoso Stachack, Ailton Jose Terezo, Cátia Nunes da Cunha, James Francis White, Haiyan Li, Marcos Antônio Soares

Research output: Contribution to journalArticle

Abstract

The present study proposes the use of endophytic fungi for mercury bioremediation in in vitro and host-associated systems. We examined mercury resistance in 32 strains of endophytic fungi grown in culture medium supplemented with toxic metal concentrations. The residual mercury concentrations were quantified after mycelial growth. Aspergillus sp. A31, Curvularia geniculata P1, Lindgomycetaceae P87, and Westerdykella sp. P71 were selected and further tested for mercury bioremediation and bioaccumulation in vitro, as well as for growth promotion of Aeschynomene fluminensis and Zea mays in the presence or absence of the metal. Aspergillus sp. A31, C. geniculata P1, Lindgomycetaceae P87 and Westerdykella sp. P71 removed up to 100% of mercury from the culture medium in a species-dependent manner and they promoted A. fluminensis and Z. mays growth in substrates containing mercury or not (Dunnett's test, p < 0.05). Lindgomycetaceae P87 and C. geniculata P1 are dark septate endophytic fungi that endophytically colonize root cells of their host plants. The increase of host biomass correlated with the reduction of soil mercury concentration due to the metal bioaccumulation in host tissues and its possible volatilization. The soil mercury concentration was decreased by 7.69% and 57.14% in A. fluminensis plants inoculated with Lindgomycetaceae P87 + Aspergillus sp. A31 and Lindgomycetaceae P87, respectively (Dunnet's test, p < 0.05). The resistance mechanisms of mercury volatilization and bioaccumulation in plant tissues mediated by these endophytic fungi can contribute to bioremediation programs. The biochemical and genetic mechanisms involved in bioaccumulation and volatilization need to be elucidated in the future.

Original languageEnglish (US)
Article number124874
JournalChemosphere
Volume240
DOIs
StatePublished - Feb 2020

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Bioaccumulation
Environmental Biodegradation
Bioremediation
Mercury (metal)
Aspergillus
Fungi
Mercury
bioremediation
Vaporization
fungus
bioaccumulation
Volatilization
Metals
Tissue
volatilization
Soils
Biomass
Zea mays
Culture Media
Soil

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

Keywords

  • Aspergillus
  • Curvularia geniculata
  • Lindgomycetaceae
  • Mercury
  • Phytoextraction
  • Westerdykella

Cite this

Pietro-Souza, W., de Campos Pereira, F., Mello, I. S., Stachack, F. F. F., Terezo, A. J., Cunha, C. N. D., ... Soares, M. A. (2020). Mercury resistance and bioremediation mediated by endophytic fungi. Chemosphere, 240, [124874]. https://doi.org/10.1016/j.chemosphere.2019.124874
Pietro-Souza, William ; de Campos Pereira, Felipe ; Mello, Ivani Souza ; Stachack, Fernando Ferrari Frutuoso ; Terezo, Ailton Jose ; Cunha, Cátia Nunes da ; White, James Francis ; Li, Haiyan ; Soares, Marcos Antônio. / Mercury resistance and bioremediation mediated by endophytic fungi. In: Chemosphere. 2020 ; Vol. 240.
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abstract = "The present study proposes the use of endophytic fungi for mercury bioremediation in in vitro and host-associated systems. We examined mercury resistance in 32 strains of endophytic fungi grown in culture medium supplemented with toxic metal concentrations. The residual mercury concentrations were quantified after mycelial growth. Aspergillus sp. A31, Curvularia geniculata P1, Lindgomycetaceae P87, and Westerdykella sp. P71 were selected and further tested for mercury bioremediation and bioaccumulation in vitro, as well as for growth promotion of Aeschynomene fluminensis and Zea mays in the presence or absence of the metal. Aspergillus sp. A31, C. geniculata P1, Lindgomycetaceae P87 and Westerdykella sp. P71 removed up to 100{\%} of mercury from the culture medium in a species-dependent manner and they promoted A. fluminensis and Z. mays growth in substrates containing mercury or not (Dunnett's test, p < 0.05). Lindgomycetaceae P87 and C. geniculata P1 are dark septate endophytic fungi that endophytically colonize root cells of their host plants. The increase of host biomass correlated with the reduction of soil mercury concentration due to the metal bioaccumulation in host tissues and its possible volatilization. The soil mercury concentration was decreased by 7.69{\%} and 57.14{\%} in A. fluminensis plants inoculated with Lindgomycetaceae P87 + Aspergillus sp. A31 and Lindgomycetaceae P87, respectively (Dunnet's test, p < 0.05). The resistance mechanisms of mercury volatilization and bioaccumulation in plant tissues mediated by these endophytic fungi can contribute to bioremediation programs. The biochemical and genetic mechanisms involved in bioaccumulation and volatilization need to be elucidated in the future.",
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Pietro-Souza, W, de Campos Pereira, F, Mello, IS, Stachack, FFF, Terezo, AJ, Cunha, CND, White, JF, Li, H & Soares, MA 2020, 'Mercury resistance and bioremediation mediated by endophytic fungi', Chemosphere, vol. 240, 124874. https://doi.org/10.1016/j.chemosphere.2019.124874

Mercury resistance and bioremediation mediated by endophytic fungi. / Pietro-Souza, William; de Campos Pereira, Felipe; Mello, Ivani Souza; Stachack, Fernando Ferrari Frutuoso; Terezo, Ailton Jose; Cunha, Cátia Nunes da; White, James Francis; Li, Haiyan; Soares, Marcos Antônio.

In: Chemosphere, Vol. 240, 124874, 02.2020.

Research output: Contribution to journalArticle

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AU - de Campos Pereira, Felipe

AU - Mello, Ivani Souza

AU - Stachack, Fernando Ferrari Frutuoso

AU - Terezo, Ailton Jose

AU - Cunha, Cátia Nunes da

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AB - The present study proposes the use of endophytic fungi for mercury bioremediation in in vitro and host-associated systems. We examined mercury resistance in 32 strains of endophytic fungi grown in culture medium supplemented with toxic metal concentrations. The residual mercury concentrations were quantified after mycelial growth. Aspergillus sp. A31, Curvularia geniculata P1, Lindgomycetaceae P87, and Westerdykella sp. P71 were selected and further tested for mercury bioremediation and bioaccumulation in vitro, as well as for growth promotion of Aeschynomene fluminensis and Zea mays in the presence or absence of the metal. Aspergillus sp. A31, C. geniculata P1, Lindgomycetaceae P87 and Westerdykella sp. P71 removed up to 100% of mercury from the culture medium in a species-dependent manner and they promoted A. fluminensis and Z. mays growth in substrates containing mercury or not (Dunnett's test, p < 0.05). Lindgomycetaceae P87 and C. geniculata P1 are dark septate endophytic fungi that endophytically colonize root cells of their host plants. The increase of host biomass correlated with the reduction of soil mercury concentration due to the metal bioaccumulation in host tissues and its possible volatilization. The soil mercury concentration was decreased by 7.69% and 57.14% in A. fluminensis plants inoculated with Lindgomycetaceae P87 + Aspergillus sp. A31 and Lindgomycetaceae P87, respectively (Dunnet's test, p < 0.05). The resistance mechanisms of mercury volatilization and bioaccumulation in plant tissues mediated by these endophytic fungi can contribute to bioremediation programs. The biochemical and genetic mechanisms involved in bioaccumulation and volatilization need to be elucidated in the future.

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KW - Phytoextraction

KW - Westerdykella

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Pietro-Souza W, de Campos Pereira F, Mello IS, Stachack FFF, Terezo AJ, Cunha CND et al. Mercury resistance and bioremediation mediated by endophytic fungi. Chemosphere. 2020 Feb;240. 124874. https://doi.org/10.1016/j.chemosphere.2019.124874