Mesencephalic astrocyte-derived neurotrophic factor enhances nigral γ-aminobutyric acid release

Chunyi Zhou; Cheng Xiao; John W. Commissiong; Krešimir Krnjević; Hong Ye Jiang

doi:10.1097/01.wnr.0000201504.23255.bc

Mesencephalic astrocyte-derived neurotrophic factor enhances nigral γ-aminobutyric acid release

Chunyi Zhou, Cheng Xiao, John W. Commissiong, Krešimir Krnjević, Hong Ye Jiang

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Mesencephalic astrocyte-derived neurotrophic factor (MANF) - one of a new class of astrocyte-derived human proteins - selectively promotes the survival of dopamine neurons of the ventral midbrain. Using the whole-cell clamp technique, we looked for acute effects of MANF on γ-aminobutyric acid type A (GABA_A) receptor-mediated inhibitory postsynaptic currents (IPSCs) in dopamine neurons of the substantia nigra pars compacta of 6 to 15-day-old rats. In slices, MANF increased the amplitude of evoked IPSCs and decreased the paired pulse ratio. In mechanically dissociated cells, MANF increased the frequency of spontaneous and miniature IPSCs, without changing their mean amplitudes; and in enzymatically dissociated neurons, MANF had no effect on currents induced by exogenous GABA. The presynaptic enhancement of GABAergic inhibition may contribute to MANF's protective action on dopamine cells.

Original language	English (US)
Pages (from-to)	293-297
Number of pages	5
Journal	NeuroReport
Volume	17
Issue number	3
DOIs	https://doi.org/10.1097/01.wnr.0000201504.23255.bc
State	Published - Feb 2006

All Science Journal Classification (ASJC) codes

Neuroscience(all)

Keywords

Dopaminergic neurons
Mesencephalic astrocyte-derived neurotrophic factor
Mesolimbic system
Neuroprotection
Substantia nigra
Whole-cell patch clamp

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10.1097/01.wnr.0000201504.23255.bc

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title = "Mesencephalic astrocyte-derived neurotrophic factor enhances nigral γ-aminobutyric acid release",

abstract = "Mesencephalic astrocyte-derived neurotrophic factor (MANF) - one of a new class of astrocyte-derived human proteins - selectively promotes the survival of dopamine neurons of the ventral midbrain. Using the whole-cell clamp technique, we looked for acute effects of MANF on γ-aminobutyric acid type A (GABAA) receptor-mediated inhibitory postsynaptic currents (IPSCs) in dopamine neurons of the substantia nigra pars compacta of 6 to 15-day-old rats. In slices, MANF increased the amplitude of evoked IPSCs and decreased the paired pulse ratio. In mechanically dissociated cells, MANF increased the frequency of spontaneous and miniature IPSCs, without changing their mean amplitudes; and in enzymatically dissociated neurons, MANF had no effect on currents induced by exogenous GABA. The presynaptic enhancement of GABAergic inhibition may contribute to MANF's protective action on dopamine cells.",

keywords = "Dopaminergic neurons, Mesencephalic astrocyte-derived neurotrophic factor, Mesolimbic system, Neuroprotection, Substantia nigra, Whole-cell patch clamp",

author = "Chunyi Zhou and Cheng Xiao and Commissiong, {John W.} and Kre{\v s}imir Krnjevi{\'c} and Jiang, {Hong Ye}",

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AU - Zhou, Chunyi

AU - Xiao, Cheng

AU - Commissiong, John W.

AU - Krnjević, Krešimir

AU - Jiang, Hong Ye

PY - 2006/2

Y1 - 2006/2

N2 - Mesencephalic astrocyte-derived neurotrophic factor (MANF) - one of a new class of astrocyte-derived human proteins - selectively promotes the survival of dopamine neurons of the ventral midbrain. Using the whole-cell clamp technique, we looked for acute effects of MANF on γ-aminobutyric acid type A (GABAA) receptor-mediated inhibitory postsynaptic currents (IPSCs) in dopamine neurons of the substantia nigra pars compacta of 6 to 15-day-old rats. In slices, MANF increased the amplitude of evoked IPSCs and decreased the paired pulse ratio. In mechanically dissociated cells, MANF increased the frequency of spontaneous and miniature IPSCs, without changing their mean amplitudes; and in enzymatically dissociated neurons, MANF had no effect on currents induced by exogenous GABA. The presynaptic enhancement of GABAergic inhibition may contribute to MANF's protective action on dopamine cells.

AB - Mesencephalic astrocyte-derived neurotrophic factor (MANF) - one of a new class of astrocyte-derived human proteins - selectively promotes the survival of dopamine neurons of the ventral midbrain. Using the whole-cell clamp technique, we looked for acute effects of MANF on γ-aminobutyric acid type A (GABAA) receptor-mediated inhibitory postsynaptic currents (IPSCs) in dopamine neurons of the substantia nigra pars compacta of 6 to 15-day-old rats. In slices, MANF increased the amplitude of evoked IPSCs and decreased the paired pulse ratio. In mechanically dissociated cells, MANF increased the frequency of spontaneous and miniature IPSCs, without changing their mean amplitudes; and in enzymatically dissociated neurons, MANF had no effect on currents induced by exogenous GABA. The presynaptic enhancement of GABAergic inhibition may contribute to MANF's protective action on dopamine cells.

KW - Dopaminergic neurons

KW - Mesencephalic astrocyte-derived neurotrophic factor

KW - Mesolimbic system

KW - Neuroprotection

KW - Substantia nigra

KW - Whole-cell patch clamp

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Mesencephalic astrocyte-derived neurotrophic factor enhances nigral γ-aminobutyric acid release

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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