Mesencephalic astrocyte-derived neurotrophic factor enhances nigral γ-aminobutyric acid release

Chunyi Zhou, Cheng Xiao, John W. Commissiong, Krešimir Krnjević, Hong Ye Jiang

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Mesencephalic astrocyte-derived neurotrophic factor (MANF) - one of a new class of astrocyte-derived human proteins - selectively promotes the survival of dopamine neurons of the ventral midbrain. Using the whole-cell clamp technique, we looked for acute effects of MANF on γ-aminobutyric acid type A (GABAA) receptor-mediated inhibitory postsynaptic currents (IPSCs) in dopamine neurons of the substantia nigra pars compacta of 6 to 15-day-old rats. In slices, MANF increased the amplitude of evoked IPSCs and decreased the paired pulse ratio. In mechanically dissociated cells, MANF increased the frequency of spontaneous and miniature IPSCs, without changing their mean amplitudes; and in enzymatically dissociated neurons, MANF had no effect on currents induced by exogenous GABA. The presynaptic enhancement of GABAergic inhibition may contribute to MANF's protective action on dopamine cells.

Original languageEnglish (US)
Pages (from-to)293-297
Number of pages5
JournalNeuroReport
Volume17
Issue number3
DOIs
StatePublished - Feb 2006

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Keywords

  • Dopaminergic neurons
  • Mesencephalic astrocyte-derived neurotrophic factor
  • Mesolimbic system
  • Neuroprotection
  • Substantia nigra
  • Whole-cell patch clamp

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