Metabonomic study on the biochemical profiles of a hydrocortisone-induced animal model

Minjun Chen, Liping Zhao, Wei Jia

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

This work describes the metabonomic study of a biochemical modification in vivo induced by high dose of hydrocortisone, which led to a unique pathologic condition similar to the 'kidney deficiency syndromes', an early stage of obesity and diabetes in traditional Chinese medicine. The methodology of the metabonomic approach consisted of GC/MS and multivariate statistical technique for the establishment of urine metabolic patterns of the treatment rats. In the study, 24-h urine was collected pre-dose and at days 1, 3, 7, and 10 post-dose after rats were injected with hydrocortisone at 1.5 mg/ 100 g. The acquired data were transferred into Matlab to be processed using principal components analysis (PCA). The results indicated that clear and consistent biochemical changes following hydrocortisone intervention under controlled conditions could be identified using chemometric analysis. The work suggests that this metabonomic approach could be used as a potentially powerful tool to investigate the biochemical changes of certain physiopathologic conditions such as metabolic syndrome, as an early diagnostic means.

Original languageEnglish (US)
Pages (from-to)2391-2396
Number of pages6
JournalJournal of Proteome Research
Volume4
Issue number6
DOIs
StatePublished - Nov 1 2005

Fingerprint

Metabolomics
Hydrocortisone
Animals
Animal Models
Rats
Urine
Chinese Traditional Medicine
Medical problems
Principal Component Analysis
Principal component analysis
Medicine
Obesity
Kidney
Therapeutics

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

Keywords

  • GC/MS
  • Hydrocortisone
  • Kidney-deficiency
  • Metabolic syndrome
  • Metabonomics

Cite this

@article{e16f8f4fa67248d7989998899508beca,
title = "Metabonomic study on the biochemical profiles of a hydrocortisone-induced animal model",
abstract = "This work describes the metabonomic study of a biochemical modification in vivo induced by high dose of hydrocortisone, which led to a unique pathologic condition similar to the 'kidney deficiency syndromes', an early stage of obesity and diabetes in traditional Chinese medicine. The methodology of the metabonomic approach consisted of GC/MS and multivariate statistical technique for the establishment of urine metabolic patterns of the treatment rats. In the study, 24-h urine was collected pre-dose and at days 1, 3, 7, and 10 post-dose after rats were injected with hydrocortisone at 1.5 mg/ 100 g. The acquired data were transferred into Matlab to be processed using principal components analysis (PCA). The results indicated that clear and consistent biochemical changes following hydrocortisone intervention under controlled conditions could be identified using chemometric analysis. The work suggests that this metabonomic approach could be used as a potentially powerful tool to investigate the biochemical changes of certain physiopathologic conditions such as metabolic syndrome, as an early diagnostic means.",
keywords = "GC/MS, Hydrocortisone, Kidney-deficiency, Metabolic syndrome, Metabonomics",
author = "Minjun Chen and Liping Zhao and Wei Jia",
year = "2005",
month = "11",
day = "1",
doi = "10.1021/pr050158o",
language = "English (US)",
volume = "4",
pages = "2391--2396",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "6",

}

Metabonomic study on the biochemical profiles of a hydrocortisone-induced animal model. / Chen, Minjun; Zhao, Liping; Jia, Wei.

In: Journal of Proteome Research, Vol. 4, No. 6, 01.11.2005, p. 2391-2396.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Metabonomic study on the biochemical profiles of a hydrocortisone-induced animal model

AU - Chen, Minjun

AU - Zhao, Liping

AU - Jia, Wei

PY - 2005/11/1

Y1 - 2005/11/1

N2 - This work describes the metabonomic study of a biochemical modification in vivo induced by high dose of hydrocortisone, which led to a unique pathologic condition similar to the 'kidney deficiency syndromes', an early stage of obesity and diabetes in traditional Chinese medicine. The methodology of the metabonomic approach consisted of GC/MS and multivariate statistical technique for the establishment of urine metabolic patterns of the treatment rats. In the study, 24-h urine was collected pre-dose and at days 1, 3, 7, and 10 post-dose after rats were injected with hydrocortisone at 1.5 mg/ 100 g. The acquired data were transferred into Matlab to be processed using principal components analysis (PCA). The results indicated that clear and consistent biochemical changes following hydrocortisone intervention under controlled conditions could be identified using chemometric analysis. The work suggests that this metabonomic approach could be used as a potentially powerful tool to investigate the biochemical changes of certain physiopathologic conditions such as metabolic syndrome, as an early diagnostic means.

AB - This work describes the metabonomic study of a biochemical modification in vivo induced by high dose of hydrocortisone, which led to a unique pathologic condition similar to the 'kidney deficiency syndromes', an early stage of obesity and diabetes in traditional Chinese medicine. The methodology of the metabonomic approach consisted of GC/MS and multivariate statistical technique for the establishment of urine metabolic patterns of the treatment rats. In the study, 24-h urine was collected pre-dose and at days 1, 3, 7, and 10 post-dose after rats were injected with hydrocortisone at 1.5 mg/ 100 g. The acquired data were transferred into Matlab to be processed using principal components analysis (PCA). The results indicated that clear and consistent biochemical changes following hydrocortisone intervention under controlled conditions could be identified using chemometric analysis. The work suggests that this metabonomic approach could be used as a potentially powerful tool to investigate the biochemical changes of certain physiopathologic conditions such as metabolic syndrome, as an early diagnostic means.

KW - GC/MS

KW - Hydrocortisone

KW - Kidney-deficiency

KW - Metabolic syndrome

KW - Metabonomics

UR - http://www.scopus.com/inward/record.url?scp=29144486833&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29144486833&partnerID=8YFLogxK

U2 - 10.1021/pr050158o

DO - 10.1021/pr050158o

M3 - Article

C2 - 16335992

AN - SCOPUS:29144486833

VL - 4

SP - 2391

EP - 2396

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 6

ER -