Method for measurement of the quaternary amine compounds paraquat and diquat in human urine using high-performance liquid chromatography-tandem mass spectrometry

Ralph D. Whitehead, M. Angela Montesano, Nayana K. Jayatilaka, Brian Buckley, Bozena Winnik, Larry L. Needham, Dana Boyd Barr

    Research output: Contribution to journalArticlepeer-review

    56 Scopus citations

    Abstract

    We have developed a highly selective and sensitive analytical method to quantify paraquat and diquat by use of high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The sample preparation includes solid phase extraction that uses weak cation exchange cartridges. These highly charged dual quaternary amines were not retained by standard reversed phase columns, but they could be adequately separated through HPLC with a HILIC column. The detection was carried out with a triple quadrupole mass spectrometer with an electrospray ionization probe in positive ion mode in multiple reaction monitoring. Repeated analysis in human urine samples spiked with low (5 ng/ml), medium (15 ng/ml), and high (30 ng/ml) concentrations of the analytes yielded relative standard deviations of less than 9%. The extraction efficiencies ranged from 77.7% to 94.2%. The limits of detection were in the range of 1 ng/ml.

    Original languageEnglish (US)
    Pages (from-to)2548-2553
    Number of pages6
    JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
    Volume878
    Issue number27
    DOIs
    StatePublished - Oct 2010

    All Science Journal Classification (ASJC) codes

    • Analytical Chemistry
    • Biochemistry
    • Clinical Biochemistry
    • Cell Biology

    Keywords

    • Diquat
    • HPLC
    • Paraquat
    • Quaternary amines
    • Tandem mass spectrometry
    • Urine

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