Method for measuring lipid mediators, proteins, and messenger RNAs from a single tissue specimen

Jessica A. Cottrell, Hsuan Ni Lin, J. Patrick O'Connor

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

This article describes a new method for extracting RNA, protein, and lipid mediators from a single tissue specimen. Specifically, mouse bone fracture callus specimens were extracted into a single solution that was processed using three different procedures to measure messenger RNA (mRNA) levels by reverse transcription-quantitative polymerase chain reaction (RTqPCR), cytokines and growth factors using an xMAP method, and lipid mediators by liquid chromatography-tandem mass spectrometry (LC-MS/MS). This method has several advantages because it decreases the number of animals necessary for experimentation, allows division of the sample from a homogeneous mixture that reduces sample variability, and uses a solution that protects the integrity of the macromolecules during storage.

Original languageEnglish (US)
Pages (from-to)34-42
Number of pages9
JournalAnalytical Biochemistry
Volume469
DOIs
StatePublished - Jan 15 2015

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Keywords

  • Bone regeneration
  • Fracture healing
  • Lipid mediator
  • Metabolomics

Fingerprint Dive into the research topics of 'Method for measuring lipid mediators, proteins, and messenger RNAs from a single tissue specimen'. Together they form a unique fingerprint.

Cite this