Abstract
Among NF-κB transcription factors, c-Rel and c-Rel-derived proteins, including v-Rel, are the only ones that have shown consistent and frank transforming activity in cell culture. In particular, viral, chicken, mouse, and human Rel proteins can rapidly transform primary chicken spleen and bone marrow cells. Overexpression of a human Rel protein missing a C-terminal transactivation domain can also enhance the transformed state of the human B-lymphoma cell line BJAB. As described in this chapter, these in vitro assays can be used to quantitatively assess the transforming activity of Rel proteins.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 427-446 |
| Number of pages | 20 |
| Journal | Methods in Molecular Biology |
| Volume | 1280 |
| DOIs | |
| State | Published - 2015 |
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
Keywords
- BJAB cells
- C-Rel
- Chicken spleen cells
- NF-kappaB
- Oncogenesis
- Transformation assay
- V-Rel