Methods for the Initiation and Maintenance of Human Endothelial Cell Culture

Patrick S. Vaccaro; Laurie B. Joseph; Lynda Titterington; Ralph E. Stephens

doi:10.1177/153857448702100604

Methods for the Initiation and Maintenance of Human Endothelial Cell Culture

Patrick S. Vaccaro, Laurie B. Joseph, Lynda Titterington, Ralph E. Stephens

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The culture of human endothelial cells for the in vitro investigation of the metabolic, synthetic, and physiologic functions of these cells has grown increas ingly common. Model systems investigating the pathogenesis of atherosclerosis are being developed. Methods for the initiation and maintenance of human en dothelial cell cultures are described. The roles of cryopreservation, microcar rier culturing, and roller bottle mass culturing are discussed.

Original language	English (US)
Pages (from-to)	391-400
Number of pages	10
Journal	Vascular and Endovascular Surgery
Volume	21
Issue number	6
DOIs	https://doi.org/10.1177/153857448702100604
State	Published - Nov 1987
Externally published	Yes

All Science Journal Classification (ASJC) codes

Surgery
Cardiology and Cardiovascular Medicine

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10.1177/153857448702100604

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title = "Methods for the Initiation and Maintenance of Human Endothelial Cell Culture",

abstract = "The culture of human endothelial cells for the in vitro investigation of the metabolic, synthetic, and physiologic functions of these cells has grown increas ingly common. Model systems investigating the pathogenesis of atherosclerosis are being developed. Methods for the initiation and maintenance of human en dothelial cell cultures are described. The roles of cryopreservation, microcar rier culturing, and roller bottle mass culturing are discussed.",

author = "Vaccaro, {Patrick S.} and Joseph, {Laurie B.} and Lynda Titterington and Stephens, {Ralph E.}",

year = "1987",

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AU - Vaccaro, Patrick S.

AU - Joseph, Laurie B.

AU - Titterington, Lynda

AU - Stephens, Ralph E.

PY - 1987/11

Y1 - 1987/11

N2 - The culture of human endothelial cells for the in vitro investigation of the metabolic, synthetic, and physiologic functions of these cells has grown increas ingly common. Model systems investigating the pathogenesis of atherosclerosis are being developed. Methods for the initiation and maintenance of human en dothelial cell cultures are described. The roles of cryopreservation, microcar rier culturing, and roller bottle mass culturing are discussed.

AB - The culture of human endothelial cells for the in vitro investigation of the metabolic, synthetic, and physiologic functions of these cells has grown increas ingly common. Model systems investigating the pathogenesis of atherosclerosis are being developed. Methods for the initiation and maintenance of human en dothelial cell cultures are described. The roles of cryopreservation, microcar rier culturing, and roller bottle mass culturing are discussed.

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Methods for the Initiation and Maintenance of Human Endothelial Cell Culture

Abstract

All Science Journal Classification (ASJC) codes

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