Methylprednisolone impairs conidial phagocytosis but does not attenuate hyphal damage by neutrophils against Exserohilum rostratum

Maria Simitsopoulou, Thomas J. Walsh, Daniela Kyrpitzi, Vidmantas Petraitis, Dimitrios P. Kontoyiannis, David S. Perlin, Emmanuel Roilides

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Exserohilum rostratum caused a multistate fungal meningitis outbreak following iatrogenic inoculation of contaminated methylprednisolone in the United States. To gain insight into the immunopathogenesis of this infection, we studied the innate host responses of human neutrophils against E. rostratum conidia and hyphae with or without methylprednisolone. The neutrophil-induced percentage fungal damage against conidia and hyphae was effector-to-target ratio dependent (≤55%). While methylprednisolone did not affect neutrophil-induced fungal damage by treatment of Exserohilum or neutrophils, it compromised phagocytosis of conidia (P < 0.05). These findings suggest that methylprednisolone-treated neutrophils may have altered phagocytic clearance of Exserohilum conidia, reducing host capacity to contain the invasive process.

Original languageEnglish (US)
Pages (from-to)189-193
Number of pages5
JournalMedical mycology
Volume53
Issue number2
DOIs
StatePublished - Oct 18 2015

All Science Journal Classification (ASJC) codes

  • Infectious Diseases

Keywords

  • Exserohilum rostratum
  • dematiaceous moulds
  • fungal meningitis
  • human polymorphonuclear leukocytes
  • innate immune response

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