Folate receptor (FR) is a typical tumor-associated antigen that has been widely used as a biomarker for the determination of cancer. A biosensing platform functionalized with folic acid (FA) is essential to FR detection because FA specifically binds FR. We demonstrate an MgZnO dual-gate thin-film transistor (MZO DGTFT) based biosensor for the sensitive determination of FA derivative, hexadecyl alkynated folic acid (HAFA). The receptor is an MZO nanostructure (MZO nano) modified sensing pad which electrically connects to the top gate of DGTFT. The bottom gate of DGTFT is used to bias the device to its optimum operation point, enabling high sensitivity. First, 11-azidoundecanoic acid (AA) was bond onto the MZO nano surface as the linker layer, resulting in negative charges to MZO nano due to carboxylic acid binding chemistry. HAFA was then immobilized on MZO nano via click reaction with AA. HAFA is a polar molecule with the positive end adjacent to AA/MZO nano, and therefore, the electrostatic condition of MZO nano is impacted again. Such changes were detected by DGTFT that displayed significant drain current variations. Fourier-transform infrared spectroscopy (FTIR) imaging confirms such successful chemical processes. This MZO DGTFT biosensor with HAFA is promising for potential applications in the detection of FR overexpressed cancer cells.
All Science Journal Classification (ASJC) codes
- Electrical and Electronic Engineering
- Click chemistry
- dual-gate thin film transistor
- hexadecyl alkynated folic acid
- magnesium zinc oxide