TY - JOUR
T1 - Mice deficient for the HNK-1 sulfotransferase show alterations in synaptic efficacy and spatial learning and memory
AU - Senn, Claudia
AU - Kutsche, Michael
AU - Saghatelyan, Armen
AU - Bösl, Michael R.
AU - Löhler, Jürgen
AU - Bartsch, Udo
AU - Morellini, Fabio
AU - Schachner, Melitta
N1 - Funding Information:
The authors are grateful to Drs. Alexander Dityatev, Astrid Rollen-hagen, and Catherina Becker for helpful discussions, Dr. Martine Albert for the HNK-1 antibodies, and Drs. Hans Welzl and Oliver Stork for helpful comments on the manuscript. This work was supported by the Deutsche Forschungsgemeinschaft (SFB 470 to M.S.).
PY - 2002
Y1 - 2002
N2 - The HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. To characterize the functional role of the HNK-1 carbohydrate in vivo, we have generated mice deficient for the HNK-1 sulfotransferase (ST). The ST-/- allele is inherited with Mendelian frequencies, and the ST-/- mice are viable and fertile. The anatomy of all major brain areas appeared histologically normal. However, basal synaptic transmission in pyramidal cells in the CA1 region of the hippocampus was increased and long-term potentiation evoked by theta-burst stimulation was reduced in ST mutants. In the water maze, ST-/- mice showed an impaired long-term memory and a poorer spatial learning when a short inter-trial interval was used. These observations indicate an essential role for the sulfate group of the HNK-1 carbohydrate in synaptic plasticity of the hippocampus.
AB - The HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. To characterize the functional role of the HNK-1 carbohydrate in vivo, we have generated mice deficient for the HNK-1 sulfotransferase (ST). The ST-/- allele is inherited with Mendelian frequencies, and the ST-/- mice are viable and fertile. The anatomy of all major brain areas appeared histologically normal. However, basal synaptic transmission in pyramidal cells in the CA1 region of the hippocampus was increased and long-term potentiation evoked by theta-burst stimulation was reduced in ST mutants. In the water maze, ST-/- mice showed an impaired long-term memory and a poorer spatial learning when a short inter-trial interval was used. These observations indicate an essential role for the sulfate group of the HNK-1 carbohydrate in synaptic plasticity of the hippocampus.
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U2 - 10.1006/mcne.2002.1142
DO - 10.1006/mcne.2002.1142
M3 - Article
C2 - 12213450
AN - SCOPUS:0036386382
SN - 1044-7431
VL - 20
SP - 712
EP - 729
JO - Molecular and Cellular Neuroscience
JF - Molecular and Cellular Neuroscience
IS - 4
ER -