Microbioreactors to manipulate oxygen tension and shear stress in the microenvironment of vascular stem and progenitor cells

Hasan E. Abaci, Raghavendra Devendra, Rohan Soman, German Drazer, Sharon Gerecht

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The dynamics of dissolved oxygen (DO) and shear stress in the vasculature microenvironment play a major role in determining the fate of stem cells in adults and during early embryonic development. In this study, we present a microbioreactor (MBR) that provides independent control over oxygen tension and shear stress in cultures of stem and progenitor cell types. We first describe the design principles and use a model-driven approach for the optimization of the MBR geometry and operating conditions prior to its fabrication and assembly. We then demonstrate the utilization of the MBR for culturing adult human endothelial progenitors, human umbilical vein endothelial cells, and human embryonic stem cell-derived smooth muscle cells under different DO and shear stress levels.

Original languageEnglish (US)
Pages (from-to)97-105
Number of pages9
JournalBiotechnology and Applied Biochemistry
Volume59
Issue number2
DOIs
StatePublished - Mar 1 2012
Externally publishedYes

Fingerprint

Blood Vessels
Shear stress
Stem Cells
Dissolved oxygen
Oxygen
Stem cells
Adult Stem Cells
Endothelial cells
Human Umbilical Vein Endothelial Cells
Smooth Muscle Myocytes
Embryonic Development
Muscle
Cells
Fabrication
Geometry
Human Embryonic Stem Cells

All Science Journal Classification (ASJC) codes

  • Drug Discovery
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering
  • Process Chemistry and Technology
  • Bioengineering
  • Molecular Medicine
  • Biotechnology

Cite this

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Microbioreactors to manipulate oxygen tension and shear stress in the microenvironment of vascular stem and progenitor cells. / Abaci, Hasan E.; Devendra, Raghavendra; Soman, Rohan; Drazer, German; Gerecht, Sharon.

In: Biotechnology and Applied Biochemistry, Vol. 59, No. 2, 01.03.2012, p. 97-105.

Research output: Contribution to journalArticle

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