Microcavity substrates casted from self-assembled microsphere monolayers for spheroid cell culture

Keyue Shen, Jungwoo Lee, Martin Yarmush, Biju Parekkadan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Multicellular spheroids are an important 3-dimensional cell culture model that reflects many key aspects of in vivo microenvironments. This paper presents a scalable, self-assembly based approach for fabricating microcavity substrates for multicellular spheroid cell culture. Hydrophobic glass microbeads were self-assembled into a tightly packed monolayer through the combined actions of surface tension, gravity, and lateral capillary forces at the water-air interface of a polymer solution. The packed bead monolayer was subsequently embedded in the dried polymer layer. The surface was used as a template for replicating microcavity substrates with perfect spherical shapes. We demonstrated the use of the substrate in monitoring the formation process of tumor spheroids, a proof-of-concept scale-up fabrication procedure into standard microplate formats, and its application in testing cancer drug responses in the context of bone marrow stromal cells. The presented technique offers a simple and effective way of forming high-density uniformly-sized spheroids without microfabrication equipment for biological and drug screening applications.

Original languageEnglish (US)
Pages (from-to)609-615
Number of pages7
JournalBiomedical Microdevices
Volume16
Issue number4
DOIs
StatePublished - Jan 1 2014

Fingerprint

Cellular Spheroids
Microcavities
Microspheres
Cell culture
Monolayers
Polymers
Cell Culture Techniques
Microtechnology
Preclinical Drug Evaluations
Surface Tension
Gravitation
Substrates
Mesenchymal Stromal Cells
Glass
Neoplasms
Microfabrication
Air
Polymer solutions
Equipment and Supplies
Self assembly

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Molecular Biology

Keywords

  • 3-Dimensional Cell Culture
  • Drug Screening
  • Microbead-Microcavity
  • Multicellular Spheroid
  • Self-Assembly

Cite this

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Microcavity substrates casted from self-assembled microsphere monolayers for spheroid cell culture. / Shen, Keyue; Lee, Jungwoo; Yarmush, Martin; Parekkadan, Biju.

In: Biomedical Microdevices, Vol. 16, No. 4, 01.01.2014, p. 609-615.

Research output: Contribution to journalArticle

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