Microfluidic image cytometry (MIC) has been developed to study phenotypes of various hPSC lines by screening several chemically defined serum/feeder-free conditions. A chemically defined hPSC culture was established using 20 ng mL-1 of bFGF on 20 μg mL-1 of Matrigel to grow hPSCs over a week in an undifferentiated state. Following hPSC culture, we conducted quantitative MIC to perform a single cell profiling of simultaneously detected protein expression (OCT4 and SSEA1). Using clustering analysis, we were able to systematically compare the characteristics of various hPSC lines in different conditions.
|Original language||English (US)|
|Number of pages||7|
|Journal||Lab on a Chip|
|State||Published - 2010|
All Science Journal Classification (ASJC) codes
- Biomedical Engineering