Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions

Ken Ichiro Kamei, Minori Ohashi, Eric Gschweng, Quinn Ho, Jane Suh, Jinghua Tang, Zeta Tak For Yu, Amander T. Clark, April D. Pyle, Michael A. Teitell, Ki Bum Lee, Owen N. Witte, Hsian Rong Tseng

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Microfluidic image cytometry (MIC) has been developed to study phenotypes of various hPSC lines by screening several chemically defined serum/feeder-free conditions. A chemically defined hPSC culture was established using 20 ng mL-1 of bFGF on 20 μg mL-1 of Matrigel to grow hPSCs over a week in an undifferentiated state. Following hPSC culture, we conducted quantitative MIC to perform a single cell profiling of simultaneously detected protein expression (OCT4 and SSEA1). Using clustering analysis, we were able to systematically compare the characteristics of various hPSC lines in different conditions.

Original languageEnglish (US)
Pages (from-to)1113-1119
Number of pages7
JournalLab on a Chip
Volume10
Issue number9
DOIs
StatePublished - 2010

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • General Chemistry
  • Biomedical Engineering

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