Microfluidic isolation of CD34-positive skin cells enables regeneration of hair and sebaceous glands in vivo

Beili Zhu, Yaakov Nahmias, Martin Yarmush, Shashi K. Murthy

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Skin stem cells resident in the bulge area of hair follicles and at the basal layer of the epidermis are multipotent and able to self-renew when transplanted into full-thickness defects in nude mice. Based on cell surface markers such as CD34 and thea6-integrin, skin stem cells can be extracted from tissuederived cell suspensions for engraftment using the gold standard cell separation technique of fluorescence-activated cell sorting (FACS). This paper describes an alternative separation method using microfluidic devices coated with degradable antibody-functionalized hydrogels. The microfluidic method allows direct injection of tissue digestate (no preprocessing tagging of cells is needed), is fast (45 minutes from injected sample to purified cells), and scalable. This method is used in this study to isolate CD34-positive (CD34+) cells from murine skin tissue digestate, and the functional capability of these cells is demonstrated by transplantation into nude mice using protocols developed by other groups for FACS-sorted cells. Specifically, the transplantation of microfluidic isolated CD34+ cells along with dermal and epidermal cells was observed to generate significant levels of hair follicles and sebaceous glands consistent with those observed previously with FACS-sorted cells.

Original languageEnglish (US)
Pages (from-to)1354-1363
Number of pages10
JournalStem Cells Translational Medicine
Volume3
Issue number11
DOIs
StatePublished - Jan 1 2014

Fingerprint

Sebaceous Glands
Microfluidics
Hair
Regeneration
Skin
Flow Cytometry
Hair Follicle
Lab-On-A-Chip Devices
Nude Mice
Stem Cells
Transplantation
Hydrogels
Cell Separation
Epidermis
Integrins
Suspensions

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Keywords

  • CD34-positive stem cells
  • Microfluidic devices
  • Nude mice
  • Skin graft site

Cite this

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Microfluidic isolation of CD34-positive skin cells enables regeneration of hair and sebaceous glands in vivo. / Zhu, Beili; Nahmias, Yaakov; Yarmush, Martin; Murthy, Shashi K.

In: Stem Cells Translational Medicine, Vol. 3, No. 11, 01.01.2014, p. 1354-1363.

Research output: Contribution to journalArticle

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