Million-fold sample stacking using on-chip isotachophoresis

Byoungsok Jung, Hao Lin, Rajiv Bharadwaj, Bijan Mohammadi, Juan G. Santiago

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a simple and robust isotachophoresis (ITP) injection method that can be integrated with microchip-based capillary electrophoresis (CE) devices to achieve millionfold sample concentration increase. This method also allows for robust sample control using either pressure-driven or electrokinetic flows, and can be performed with simple, offthe- shelf chips. We have also developed a fast numerical model for ITP that leverages an area-averaged formulation of the general equations. The model captures dispersion effects, is valid for multiple species, and can be used to optimize sample concentration processes.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages617-619
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - 2005
Externally publishedYes
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
Country/TerritoryUnited States
CityBoston, MA
Period10/9/0510/13/05

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Keywords

  • Isotachophoresis
  • On-chip capillary electrophoresis
  • Sample stacking

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