Mode of action of diterpene and characterization of related metabolites from the soft coral, Xeniaelongata

Eric H. Andrianasolo; Liti Haramaty; Eileen White; Richard Lutz; Paul Falkowski

doi:10.3390/md12021102

Mode of action of diterpene and characterization of related metabolites from the soft coral, Xeniaelongata

Eric H. Andrianasolo, Liti Haramaty, Eileen White, Richard Lutz, Paul Falkowski

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Chemical and biological investigation of the cultured marine soft coral Xenia elongata led to the isolation of two new diterpenes (2, 3). Their structures were elucidated using a combination of NMR and mass spectrometry. Biological evaluations and assessments were determined using the specific apoptosis induction assay based on genetically engineered mammalian cell line D3 deficient in Bak and Bax and derived from a mouse epithelial cell. The diterpenes induce apoptosis in low micromolar concentrations. The results indicate that the previously isolated compound (1) affects cell in a manner similar to that of HSP90 and HDAC inhibitors and in a manner opposite of PI3 kinase/mTOR inhibitors. Compound (3) inhibits selectively HDAC6 in high micromolar concentrations.

Original language	English (US)
Pages (from-to)	1102-1115
Number of pages	14
Journal	Marine Drugs
Volume	12
Issue number	2
DOIs	https://doi.org/10.3390/md12021102
State	Published - Feb 2014

All Science Journal Classification (ASJC) codes

Drug Discovery

Keywords

Bioactive compounds and bioproducts
Drug discovery and development
Marine biotechnology
Structural and functional characterization of marine drug

Access to Document

10.3390/md12021102

Cite this

@article{706b973f45e74636a1e19a5352c26e62,

title = "Mode of action of diterpene and characterization of related metabolites from the soft coral, Xeniaelongata",

abstract = "Chemical and biological investigation of the cultured marine soft coral Xenia elongata led to the isolation of two new diterpenes (2, 3). Their structures were elucidated using a combination of NMR and mass spectrometry. Biological evaluations and assessments were determined using the specific apoptosis induction assay based on genetically engineered mammalian cell line D3 deficient in Bak and Bax and derived from a mouse epithelial cell. The diterpenes induce apoptosis in low micromolar concentrations. The results indicate that the previously isolated compound (1) affects cell in a manner similar to that of HSP90 and HDAC inhibitors and in a manner opposite of PI3 kinase/mTOR inhibitors. Compound (3) inhibits selectively HDAC6 in high micromolar concentrations.",

keywords = "Bioactive compounds and bioproducts, Drug discovery and development, Marine biotechnology, Structural and functional characterization of marine drug",

author = "Andrianasolo, {Eric H.} and Liti Haramaty and Eileen White and Richard Lutz and Paul Falkowski",

year = "2014",

month = feb,

doi = "10.3390/md12021102",

language = "English (US)",

volume = "12",

pages = "1102--1115",

journal = "Marine Drugs",

issn = "1660-3397",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "2",

}

TY - JOUR

T1 - Mode of action of diterpene and characterization of related metabolites from the soft coral, Xeniaelongata

AU - Andrianasolo, Eric H.

AU - Haramaty, Liti

AU - White, Eileen

AU - Lutz, Richard

AU - Falkowski, Paul

PY - 2014/2

Y1 - 2014/2

N2 - Chemical and biological investigation of the cultured marine soft coral Xenia elongata led to the isolation of two new diterpenes (2, 3). Their structures were elucidated using a combination of NMR and mass spectrometry. Biological evaluations and assessments were determined using the specific apoptosis induction assay based on genetically engineered mammalian cell line D3 deficient in Bak and Bax and derived from a mouse epithelial cell. The diterpenes induce apoptosis in low micromolar concentrations. The results indicate that the previously isolated compound (1) affects cell in a manner similar to that of HSP90 and HDAC inhibitors and in a manner opposite of PI3 kinase/mTOR inhibitors. Compound (3) inhibits selectively HDAC6 in high micromolar concentrations.

AB - Chemical and biological investigation of the cultured marine soft coral Xenia elongata led to the isolation of two new diterpenes (2, 3). Their structures were elucidated using a combination of NMR and mass spectrometry. Biological evaluations and assessments were determined using the specific apoptosis induction assay based on genetically engineered mammalian cell line D3 deficient in Bak and Bax and derived from a mouse epithelial cell. The diterpenes induce apoptosis in low micromolar concentrations. The results indicate that the previously isolated compound (1) affects cell in a manner similar to that of HSP90 and HDAC inhibitors and in a manner opposite of PI3 kinase/mTOR inhibitors. Compound (3) inhibits selectively HDAC6 in high micromolar concentrations.

KW - Bioactive compounds and bioproducts

KW - Drug discovery and development

KW - Marine biotechnology

KW - Structural and functional characterization of marine drug

UR - http://www.scopus.com/inward/record.url?scp=84896712703&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896712703&partnerID=8YFLogxK

U2 - 10.3390/md12021102

DO - 10.3390/md12021102

M3 - Article

C2 - 24562393

AN - SCOPUS:84896712703

SN - 1660-3397

VL - 12

SP - 1102

EP - 1115

JO - Marine Drugs

JF - Marine Drugs

IS - 2

ER -

Mode of action of diterpene and characterization of related metabolites from the soft coral, Xeniaelongata

Abstract

All Science Journal Classification (ASJC) codes

Keywords

Access to Document

Other files and links

Fingerprint

Cite this