Model of unidirectional transluminal gene transfer

Marco A. Arap; Johanna Lahdenranta; Amin Hajitou; Frank C. Marini; Christopher G. Wood; Kenneth C. Wright; Juan Fueyo; Wadih Arap; Renata Pasqualini

doi:10.1016/j.ymthe.2003.11.016

Model of unidirectional transluminal gene transfer

Marco A. Arap, Johanna Lahdenranta, Amin Hajitou, Frank C. Marini, Christopher G. Wood, Kenneth C. Wright, Juan Fueyo, Wadih Arap, Renata Pasqualini

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Gene transfer assays in vitro are poor indicators of transduction efficacy observed in vivo. We designed and optimized an intermediate model for assessing and quantifying unidirectional transduction ex vivo. The model enables simultaneous transmucosal evaluation of up to 96 different variables under the same tissue conditions. We show that the model is versatile and suitable for use with different vectors (adenovirus and AAV), different reporter genes (β-galactosidase and green fluorescent protein), and viscera with various tissue features such as peritoneum and urothelium. Ex vivo transduction assays may correlate better with in vivo gene transfer results. Because the experimental model described here can be performed in small samples, it may enable translational applications in tissues of human origin.

Original language	English (US)
Pages (from-to)	305-310
Number of pages	6
Journal	Molecular Therapy
Volume	9
Issue number	2
DOIs	https://doi.org/10.1016/j.ymthe.2003.11.016
State	Published - Feb 2004
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Medicine
Molecular Biology
Genetics
Pharmacology
Drug Discovery

Keywords

AAV
Adenovirus
Ex vivo model
Gene transfer
Green fluorescent protein
Transduction
β-galactosidase

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10.1016/j.ymthe.2003.11.016

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title = "Model of unidirectional transluminal gene transfer",

abstract = "Gene transfer assays in vitro are poor indicators of transduction efficacy observed in vivo. We designed and optimized an intermediate model for assessing and quantifying unidirectional transduction ex vivo. The model enables simultaneous transmucosal evaluation of up to 96 different variables under the same tissue conditions. We show that the model is versatile and suitable for use with different vectors (adenovirus and AAV), different reporter genes (β-galactosidase and green fluorescent protein), and viscera with various tissue features such as peritoneum and urothelium. Ex vivo transduction assays may correlate better with in vivo gene transfer results. Because the experimental model described here can be performed in small samples, it may enable translational applications in tissues of human origin.",

keywords = "AAV, Adenovirus, Ex vivo model, Gene transfer, Green fluorescent protein, Transduction, β-galactosidase",

author = "Arap, {Marco A.} and Johanna Lahdenranta and Amin Hajitou and Marini, {Frank C.} and Wood, {Christopher G.} and Wright, {Kenneth C.} and Juan Fueyo and Wadih Arap and Renata Pasqualini",

note = "Funding Information: We thank Dr. Dimitry Shayakhmetov for the gift of Ad-LacZ vector and Dr. Corazon D. Bucana for assistance with confocal microscopy. This work was supported by NCI Grants CA91846 (to W.A.) and CA88106 (to R.P.) and by awards from Angel Works, the Gillson–Longenbaugh Foundation, and the V Foundation (all to R.P. and W.A.).",

year = "2004",

month = feb,

doi = "10.1016/j.ymthe.2003.11.016",

language = "English (US)",

volume = "9",

pages = "305--310",

journal = "Molecular Therapy",

issn = "1525-0016",

publisher = "Cell Press",

number = "2",

}

TY - JOUR

T1 - Model of unidirectional transluminal gene transfer

AU - Arap, Marco A.

AU - Lahdenranta, Johanna

AU - Hajitou, Amin

AU - Marini, Frank C.

AU - Wood, Christopher G.

AU - Wright, Kenneth C.

AU - Fueyo, Juan

AU - Arap, Wadih

AU - Pasqualini, Renata

N1 - Funding Information: We thank Dr. Dimitry Shayakhmetov for the gift of Ad-LacZ vector and Dr. Corazon D. Bucana for assistance with confocal microscopy. This work was supported by NCI Grants CA91846 (to W.A.) and CA88106 (to R.P.) and by awards from Angel Works, the Gillson–Longenbaugh Foundation, and the V Foundation (all to R.P. and W.A.).

PY - 2004/2

Y1 - 2004/2

N2 - Gene transfer assays in vitro are poor indicators of transduction efficacy observed in vivo. We designed and optimized an intermediate model for assessing and quantifying unidirectional transduction ex vivo. The model enables simultaneous transmucosal evaluation of up to 96 different variables under the same tissue conditions. We show that the model is versatile and suitable for use with different vectors (adenovirus and AAV), different reporter genes (β-galactosidase and green fluorescent protein), and viscera with various tissue features such as peritoneum and urothelium. Ex vivo transduction assays may correlate better with in vivo gene transfer results. Because the experimental model described here can be performed in small samples, it may enable translational applications in tissues of human origin.

AB - Gene transfer assays in vitro are poor indicators of transduction efficacy observed in vivo. We designed and optimized an intermediate model for assessing and quantifying unidirectional transduction ex vivo. The model enables simultaneous transmucosal evaluation of up to 96 different variables under the same tissue conditions. We show that the model is versatile and suitable for use with different vectors (adenovirus and AAV), different reporter genes (β-galactosidase and green fluorescent protein), and viscera with various tissue features such as peritoneum and urothelium. Ex vivo transduction assays may correlate better with in vivo gene transfer results. Because the experimental model described here can be performed in small samples, it may enable translational applications in tissues of human origin.

KW - AAV

KW - Adenovirus

KW - Ex vivo model

KW - Gene transfer

KW - Green fluorescent protein

KW - Transduction

KW - β-galactosidase

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DO - 10.1016/j.ymthe.2003.11.016

M3 - Article

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AN - SCOPUS:1342290207

SN - 1525-0016

VL - 9

SP - 305

EP - 310

JO - Molecular Therapy

JF - Molecular Therapy

IS - 2

ER -

Model of unidirectional transluminal gene transfer

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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