Modulation of cardiac Ca2+ channels by IGF1

Michele L. Solem, Andrew P. Thomas

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Although there are several reports on the regulation of neuronal and skeletal muscle voltage-sensitive calcium channels by IGF1, the effects of short-term IGF1 exposure on cardiac Ca2+ channels have not been described. We measured the activity of nitrendipine-sensitive Ca2+ channels of intact cardiac myocytes in the presence of IGF1 by monitoring unidirectional Mn2+ influx measured as the quench of cytosolic fura-2 in electrically stimulated or K+-depolarized cells. Maximal channel activation was observed after 10 min of preincubation with IGF1, which gave an increase of 216 +/- 25%. Treatment with the protein kinase C inhibitors bisindolybmaleimide I and chelerythline mimicked the augmentation effect of IGF1, whereas PMA blocked enhancement of Mn2+ influx by IGF1. These results demonstrate that acute IGF1 augments dihydropyridine-sensitive sarcolemmal Ca2+ channel activity and that protein kinase C may contribute to the regulation of cardiac Ca2+ channels by IGF1.

Original languageEnglish (US)
Pages (from-to)151-155
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume252
Issue number1
DOIs
StatePublished - Nov 9 1998

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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