Modulation of hemocoagulative response by lead nitrate

M. A. Sohail, D. Kristol, C. R. Spillert, R. R. Arora

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Introduction of a lead ion in the form of lead nitrate has been found to induce coagulopathy in human blood. A marked toxic action on the normal hemocoagulative mechanism, under a background of lead intoxication, is manifested through an increase of clotting time for lead treated blood. The in vitro study carried out with citrated human blood samples (n=12) utilized celite as a procoagulant in both the control samples and the lead treated blood samples. Various 500 μl samples of blood were treated with varying molar concentrations of lead nitrate. As a substitute for the lead nitrate solution, the control was treated with 0.1% saline solution. It should be noted that at this concentration, saline solution does not alter the coagulation time of blood. Following the incubation of the samples for 10 min. at 37°C, 200 μl of blood was taken from each sample and recalcified by 20 μl of 0.1 M CaCl2 to initiate clotting for measuring the coagulation time. Results thus obtained indicated a direct correlation between Pb(NO3)2 addition and coagulation time for each sample. Furthermore, anticoagulant activity was shown to increase with higher concentrations of Pb(NO3)2.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE 29th Annual Northeast Bioengineering Conference
EditorsStanley Reisman, Richard Foulds, Bruno Mantilla
PublisherInstitute of Electrical and Electronics Engineers Inc.
Number of pages2
ISBN (Electronic)0780377672
StatePublished - 2003
Event29th IEEE Annual Northeast Bioengineering Conference, NEBC 2003 - Newark, United States
Duration: Mar 22 2003Mar 23 2003

Publication series

NameProceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
ISSN (Print)1071-121X
ISSN (Electronic)2160-7001


Other29th IEEE Annual Northeast Bioengineering Conference, NEBC 2003
Country/TerritoryUnited States

All Science Journal Classification (ASJC) codes

  • Bioengineering


  • Blood
  • Coagulation
  • Diseases
  • Humans
  • Instruments
  • Lead
  • Medical treatment
  • Proteins
  • Temperature
  • Time measurement


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