Molecular analysis of a recombinant M-MuLV/RaLV retrovirus

Rodrigo A. Villanueva; Stephen Campbell; Monica J. Roth

doi:10.1016/S0042-6822(03)00518-X

Molecular analysis of a recombinant M-MuLV/RaLV retrovirus

Rodrigo A. Villanueva, Stephen Campbell, Monica J. Roth

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2 Scopus citations

Abstract

A replication-competent retrovirus was isolated from Rat1 cells after stable transfection of a defective Moloney murine leukemia virus (M-MuLV) provirus bearing mutations in two conserved cysteines of the TM protein. Immunoprecipitation of the viral proteins indicated the infectious virus is not related to M-MuLV. Electron microscopy of budding virions revealed a mammalian type C virus. The host range of the virus is limited to rat cells. N-terminal sequence analysis of the capsid-associated protein identified the virus to be related to rat leukemia virus (RaLV). Analysis of the cloned sequences indicated a recombinant provirus with a genetic organization in which the leader region and open reading frames of the endogenous RaLV are flanked by identical M-MuLV LTRs at both ends. These results highlight the effects of exogenous viral infection on endogenous viruses.

Original language	English (US)
Pages (from-to)	195-208
Number of pages	14
Journal	Virology
Volume	315
Issue number	1
DOIs	https://doi.org/10.1016/S0042-6822(03)00518-X
State	Published - Oct 10 2003

All Science Journal Classification (ASJC) codes

Virology

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10.1016/S0042-6822(03)00518-X

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title = "Molecular analysis of a recombinant M-MuLV/RaLV retrovirus",

abstract = "A replication-competent retrovirus was isolated from Rat1 cells after stable transfection of a defective Moloney murine leukemia virus (M-MuLV) provirus bearing mutations in two conserved cysteines of the TM protein. Immunoprecipitation of the viral proteins indicated the infectious virus is not related to M-MuLV. Electron microscopy of budding virions revealed a mammalian type C virus. The host range of the virus is limited to rat cells. N-terminal sequence analysis of the capsid-associated protein identified the virus to be related to rat leukemia virus (RaLV). Analysis of the cloned sequences indicated a recombinant provirus with a genetic organization in which the leader region and open reading frames of the endogenous RaLV are flanked by identical M-MuLV LTRs at both ends. These results highlight the effects of exogenous viral infection on endogenous viruses.",

author = "Villanueva, {Rodrigo A.} and Stephen Campbell and Roth, {Monica J.}",

note = "Funding Information: This research is supported by NIH Grant R01 CA49932 to M.J.R. We thank Kunio Nagashima for EM technical support and Ann Thomas for technical assistance.",

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T1 - Molecular analysis of a recombinant M-MuLV/RaLV retrovirus

AU - Villanueva, Rodrigo A.

AU - Campbell, Stephen

AU - Roth, Monica J.

N1 - Funding Information: This research is supported by NIH Grant R01 CA49932 to M.J.R. We thank Kunio Nagashima for EM technical support and Ann Thomas for technical assistance.

PY - 2003/10/10

Y1 - 2003/10/10

N2 - A replication-competent retrovirus was isolated from Rat1 cells after stable transfection of a defective Moloney murine leukemia virus (M-MuLV) provirus bearing mutations in two conserved cysteines of the TM protein. Immunoprecipitation of the viral proteins indicated the infectious virus is not related to M-MuLV. Electron microscopy of budding virions revealed a mammalian type C virus. The host range of the virus is limited to rat cells. N-terminal sequence analysis of the capsid-associated protein identified the virus to be related to rat leukemia virus (RaLV). Analysis of the cloned sequences indicated a recombinant provirus with a genetic organization in which the leader region and open reading frames of the endogenous RaLV are flanked by identical M-MuLV LTRs at both ends. These results highlight the effects of exogenous viral infection on endogenous viruses.

AB - A replication-competent retrovirus was isolated from Rat1 cells after stable transfection of a defective Moloney murine leukemia virus (M-MuLV) provirus bearing mutations in two conserved cysteines of the TM protein. Immunoprecipitation of the viral proteins indicated the infectious virus is not related to M-MuLV. Electron microscopy of budding virions revealed a mammalian type C virus. The host range of the virus is limited to rat cells. N-terminal sequence analysis of the capsid-associated protein identified the virus to be related to rat leukemia virus (RaLV). Analysis of the cloned sequences indicated a recombinant provirus with a genetic organization in which the leader region and open reading frames of the endogenous RaLV are flanked by identical M-MuLV LTRs at both ends. These results highlight the effects of exogenous viral infection on endogenous viruses.

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ER -

Molecular analysis of a recombinant M-MuLV/RaLV retrovirus

Abstract

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