TY - JOUR
T1 - Molecular characterization of sulfate-reducing bacteria in anaerobic hydrocarbon-degrading consortia and pure cultures using the dissimilatory sulfite reductase (dsrAB) genes
AU - Pérez-Jiménez, J. R.
AU - Young, L. Y.
AU - Kerkhof, L. J.
N1 - Funding Information:
This work was supported in part by Grant from the Office of Naval Research (#N00014-99-1-0) to L.Y.Y. and L.J.K., Grant from the National Science Foundation (#9872024) to L.J.K., and by an Initiative for Minority Student Development Award from the National Institutes of Health (#R25 BM55146) for J.R.P.-J. We are grateful to Drs. Alfred Boyle, Craig Phelps, Chi Ming So, Elise R. Sullivan and Xiaoming Zhang for cultures of the novel sulfidogenic degraders. We would also like to thank Dr. Elise R. Sullivan for helpful discussions and review of the manuscript.
PY - 2001
Y1 - 2001
N2 - The characterization of sulfate-reducing bacteria (SRBs) is presented using the dissimilatory sulfite reductase (dsrAB) gene from various samples capable of mineralizing petroleum components. These samples include several novel, sulfidogenic pure cultures which degrade alkanes, toluene, and tribromophenol. Additionally, we have sulfidogenic consortia which re-mineralize benzene, naphthalene, 2-methylnaphthalene, and phenanthrene as a sole carbon source. In this study, 22 new dsrAB genes were cloned and sequenced. The dsrAB genes from our pollutant-degrading cultures or consortia were distributed among known SRBs and previously described dsrAB environmental clones, suggesting that many biodegradative SRBs are phylogenetically distinct and geographically wide spread. Specifically, the same dsrAB gene was discovered in independently established consortia capable of benzene, phenanthrene, and methylnaphthalene degradation, indicating that this particular SRB may be a key player in anaerobic degradation of hydrocarbons in the environment.
AB - The characterization of sulfate-reducing bacteria (SRBs) is presented using the dissimilatory sulfite reductase (dsrAB) gene from various samples capable of mineralizing petroleum components. These samples include several novel, sulfidogenic pure cultures which degrade alkanes, toluene, and tribromophenol. Additionally, we have sulfidogenic consortia which re-mineralize benzene, naphthalene, 2-methylnaphthalene, and phenanthrene as a sole carbon source. In this study, 22 new dsrAB genes were cloned and sequenced. The dsrAB genes from our pollutant-degrading cultures or consortia were distributed among known SRBs and previously described dsrAB environmental clones, suggesting that many biodegradative SRBs are phylogenetically distinct and geographically wide spread. Specifically, the same dsrAB gene was discovered in independently established consortia capable of benzene, phenanthrene, and methylnaphthalene degradation, indicating that this particular SRB may be a key player in anaerobic degradation of hydrocarbons in the environment.
KW - Alkane degradation
KW - Biodegradation
KW - Dissimilatory sulfite reductase
KW - Monoaromatic hydrocarbon degradation
KW - Polyaromatic hydrocarbon degradation
KW - Sulfate-reducing bacteria
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U2 - 10.1016/S0168-6496(00)00123-9
DO - 10.1016/S0168-6496(00)00123-9
M3 - Article
AN - SCOPUS:0035072290
SN - 0168-6496
VL - 35
SP - 145
EP - 150
JO - FEMS Microbiology Ecology
JF - FEMS Microbiology Ecology
IS - 2
ER -