Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation, characterization and regulation of the SPO7 sporulation gene

Wendy Whyte, Leila H. Keopp, Jacque Lamb, Joan C. Crowley, David B. Kaback

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

SPO7 is one of several previously identified genes from the yeast Saccharomyces cerevisiae that is required for sporulation but not for vegetative growth. The SPO7 gene has been cloned by functional complementation and physically mapped 15-16 kb to the left of CENI. Gene-disruption experimenta confirmed that the cloned gene was the bona ide SPO7 gene. SPO7 codes for a 0.95-kb transcript that is expressed at approximately the same level in both vegetative and sporulating cells. The gene was sequenced and has the capacity to encode a 259-amino acid protein that does not appear to be related to other known proteins.

Original languageEnglish (US)
Pages (from-to)65-72
Number of pages8
JournalGene
Volume95
Issue number1
DOIs
StatePublished - Oct 30 1990

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • Recombinant DNA
  • antimutator
  • meiosis
  • yeast

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