Molecular Cloning of Mammalian 28,000 Mr Vitamin D-Dependent Calcium Binding Protein (Calbindin-D28K): Expression of Calbindin-D28K RNAs in Rodent Brain and Kidney

Teresa L. Wood, Yutaka Kobayashi, Gretchen Frantz, Samuel Varghese, Sylvia Christakos, Allan J. Tobin

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Abstract

We report the isolation of a cloned cDNA for the mammalian 28,000 Mr vitamin D-dependent calcium binding protein (calbindin-D28K; CaBP28K) by immunological screening of a λgt11 bacterial expression library. The library contained cDNAs copied from poly(A)RNA of adult mouse cerebellum. We confirmed the identity of the CaBP28K cDNA by comparing its DNA sequence with that of chick CaBP28K cDNA. In the coding region, 79% of the mouse cDNA sequence was identical to the reported sequence of CaBP28K cDNA derived from chicken intestine. Rat brain and kidney each contain three species of poly(A)RNA that hybridize to CaBP28K cDNA–a major species of 1.9 kb, and rarer components of 2.8 kb and 3.2 kb. All three RNAs appear to be transcribed from a single gene. The ratios of these CaBP28K RNAs were the same in brain and kidney. In the cerebellum, in situ hybridization reveals that CaBP28K RNAs are confined to Purkinje neurons.

Original languageEnglish (US)
Pages (from-to)585-593
Number of pages9
JournalDNA
Volume7
Issue number9
DOIs
StatePublished - Nov 1988

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

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