TY - JOUR
T1 - Molecular method for discrimination between Francisella tularensis and Francisella-like endosymbionts
AU - Escudero, Raquel
AU - Toledo, A.
AU - Gil, Horacio
AU - Kovácsová, Katarina
AU - Rodríguez-Vargas, Manuela
AU - Jado, Isabel
AU - García-Amil, Cristina
AU - Lobo, Bruno
AU - Bhide, Mangesh
AU - Anda, Pedro
PY - 2008/9
Y1 - 2008/9
N2 - Environmental studies on the distribution of Francisella spp. are hampered by the frequency of Francisella-like endosymbionts that can produce a misleading positive result. A new, efficient molecular method for detection of Francisella tularensis and its discrimination from Francisella-like endosymbionts, as well as two variants associated with human disease (unusual F. tularensis strain FnSp1 and F. tularensis subsp. novicida-like strain 3523), is described. The method is highly specific and sensitive, detecting up to one plasmid copy or 10 genome equivalents.
AB - Environmental studies on the distribution of Francisella spp. are hampered by the frequency of Francisella-like endosymbionts that can produce a misleading positive result. A new, efficient molecular method for detection of Francisella tularensis and its discrimination from Francisella-like endosymbionts, as well as two variants associated with human disease (unusual F. tularensis strain FnSp1 and F. tularensis subsp. novicida-like strain 3523), is described. The method is highly specific and sensitive, detecting up to one plasmid copy or 10 genome equivalents.
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U2 - 10.1128/JCM.00275-08
DO - 10.1128/JCM.00275-08
M3 - Article
C2 - 18650358
AN - SCOPUS:53649099959
SN - 0095-1137
VL - 46
SP - 3139
EP - 3143
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 9
ER -