@inbook{68578a076474453bb3594c3034f52055,
title = "Monitoring Calcium Fluxes and Lysosome Exocytosis During Pyroptosis",
abstract = "Inflammasome-mediated activation of inflammatory caspases (caspase-1, caspase-4, caspase-5, caspase-11) initiates a cascade of cellular events that lead to proinflammatory cell death, or pyroptosis. Proteolytic cleavage of gasdermin D results in the formation of transmembrane pores that allow the release of mature cytokines IL-1β and IL-18. Gasdermin pores also allow calcium influx through the plasma membrane, triggering the fusion of lysosomal compartments with the cell surface and release of their contents into the extracellular milieu in a process termed lysosome exocytosis. This chapter outlines methods for measuring calcium flux, lysosome exocytosis, and membrane disruption after inflammatory caspase activation.",
keywords = "Calcium, Gasdermin D, Inflammation, Inflammatory caspases, Lysosome exocytosis, Pyroptosis",
author = "Loomis, {Wendy P.} and Tessa Bergsbaken",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-3040-2_14",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "171--178",
booktitle = "Methods in Molecular Biology",
}