Monoclonal antibodies used as probes for the structural organization of the central region of fibronectin

M. Dziadek; H. Richter; M. Schachner; R. Timpl

doi:10.1016/0014-5793(82)80629-7

Monoclonal antibodies used as probes for the structural organization of the central region of fibronectin

M. Dziadek, H. Richter, M. Schachner, R. Timpl

School of Arts and Sciences, Cell Biology & Neuroscience

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Two monoclonal mouse antibodies against human plasma fibronectin were compared in their reactivity for proteolytic fragments of the antigen by enzyme immunoassay and immunoblotting. These antibodies were shown to react with two different structures within a short segment (about 30 kDa) located about one-third away from the C-terminus of the fibronectin chains.

Original language	English (US)
Pages (from-to)	321-325
Number of pages	5
Journal	FEBS Letters
Volume	155
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(82)80629-7
State	Published - May 8 1983

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Keywords

Central region structure
Fibronectin
Immunoblotting
Monoclonal antibody

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10.1016/0014-5793(82)80629-7

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title = "Monoclonal antibodies used as probes for the structural organization of the central region of fibronectin",

abstract = "Two monoclonal mouse antibodies against human plasma fibronectin were compared in their reactivity for proteolytic fragments of the antigen by enzyme immunoassay and immunoblotting. These antibodies were shown to react with two different structures within a short segment (about 30 kDa) located about one-third away from the C-terminus of the fibronectin chains.",

keywords = "Central region structure, Fibronectin, Immunoblotting, Monoclonal antibody",

author = "M. Dziadek and H. Richter and M. Schachner and R. Timpl",

note = "Funding Information: This work was supported by grants from the Deutsche Forschungsgemeinschaf(tT i 95/5 and Ho 740).M .D. was in receipto f a fellowshipf rom the European Molecular Biology Organization.",

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doi = "10.1016/0014-5793(82)80629-7",

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T1 - Monoclonal antibodies used as probes for the structural organization of the central region of fibronectin

AU - Dziadek, M.

AU - Richter, H.

AU - Schachner, M.

AU - Timpl, R.

N1 - Funding Information: This work was supported by grants from the Deutsche Forschungsgemeinschaf(tT i 95/5 and Ho 740).M .D. was in receipto f a fellowshipf rom the European Molecular Biology Organization.

PY - 1983/5/8

Y1 - 1983/5/8

N2 - Two monoclonal mouse antibodies against human plasma fibronectin were compared in their reactivity for proteolytic fragments of the antigen by enzyme immunoassay and immunoblotting. These antibodies were shown to react with two different structures within a short segment (about 30 kDa) located about one-third away from the C-terminus of the fibronectin chains.

AB - Two monoclonal mouse antibodies against human plasma fibronectin were compared in their reactivity for proteolytic fragments of the antigen by enzyme immunoassay and immunoblotting. These antibodies were shown to react with two different structures within a short segment (about 30 kDa) located about one-third away from the C-terminus of the fibronectin chains.

KW - Central region structure

KW - Fibronectin

KW - Immunoblotting

KW - Monoclonal antibody

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ER -

Monoclonal antibodies used as probes for the structural organization of the central region of fibronectin

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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