Monoclonal IgM in a patient with paraproteinemic polyneuropathy binds to gangliosides containing disialosyl groups

Amjad A. Ilyas, Richard H. Quarles, Marinos C. Dalakas, Peter H. Fishman, Roscoe O. Brady

Research output: Contribution to journalArticle

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Abstract

Monoclonal IgM K from a patient with polyneuropathy associated with paraproteinemia was found to bind to several polysialogangliosides. Binding of IgM to gangliosides was shown by enzyme‐linked immunosorbent assay (ELISA) and by overlaying thin‐layer chromatograms of human brain and peripheral nerve gangliosides with the patient's serum followed by radioiodinated goat antihuman IgM. The latter technique showed that the IgM paraprotein reacted with a number of gangliosides. In an ELISA the IgM paraprotein reacted strongly with GD2, GD3, GD1b, and GT1b, but not with GM1, GM3, and GD1a. Thus, the epitope for the patient's IgM paraprotein appears to involve the disialosyl configurations.

Original languageEnglish (US)
Pages (from-to)655-659
Number of pages5
JournalAnnals of Neurology
Volume18
Issue number6
DOIs
StatePublished - Dec 1985

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

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