Morphological mutant in the entomopathogenic nematode, heterorhabditis bacteriophora

F. R. Rahimi; T. R. McGuire; R. Gaugler

doi:10.1093/oxfordjournals.jhered.a111374

Morphological mutant in the entomopathogenic nematode, heterorhabditis bacteriophora

F. R. Rahimi, T. R. McGuire, R. Gaugler

School of Environmental and Biological Sciences, Entomology

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Heterorhabditis bacteriophora is an entomopathogenic nematode that has potential as a biological control agent. As a first step in the genetic analysis of this nematode, we induced and isolated a short nematode (dumpy) from the F₂progeny of an EMS mutagenized nematode. Phenotypically, the dumpy adult is 60% shorter than the wild type. There is almost no longitudinal growth after the infective juvenile stage. Dumpy is completely recessive with 100% penetrance and uniform expressivity. When F₁progeny were allowed to self, F₂progeny segregated wild: dumpy in a 15:1 ratio (χ²= 0.634,.5 >P >.2). This dumpy mutation (Rhdpy) complements another dumpy mutation isolated in Israel and is the second gene known for this species.

Original language	English (US)
Pages (from-to)	475-478
Number of pages	4
Journal	Journal of Heredity
Volume	84
Issue number	6
DOIs	https://doi.org/10.1093/oxfordjournals.jhered.a111374
State	Published - Nov 1993

All Science Journal Classification (ASJC) codes

Biotechnology
Molecular Biology
Genetics
Genetics(clinical)

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title = "Morphological mutant in the entomopathogenic nematode, heterorhabditis bacteriophora",

abstract = "Heterorhabditis bacteriophora is an entomopathogenic nematode that has potential as a biological control agent. As a first step in the genetic analysis of this nematode, we induced and isolated a short nematode (dumpy) from the F2progeny of an EMS mutagenized nematode. Phenotypically, the dumpy adult is 60% shorter than the wild type. There is almost no longitudinal growth after the infective juvenile stage. Dumpy is completely recessive with 100% penetrance and uniform expressivity. When F1progeny were allowed to self, F2progeny segregated wild: dumpy in a 15:1 ratio (χ2= 0.634,.5 >P >.2). This dumpy mutation (Rhdpy) complements another dumpy mutation isolated in Israel and is the second gene known for this species.",

author = "Rahimi, {F. R.} and McGuire, {T. R.} and R. Gaugler",

note = "Funding Information: From the Department of Biological Science, Rutgers the State University of New Jersey, Piscataway (Rahimi and McGuire), and the Department of Entomology, Rutgers the State University of New Jersey, New Brunswick (Gaugler). The authors wish to thank John L. Grazul from Rutgers University, Bureau of Biological Research Electron Microscope Facility, Piscataway, New Jersey, for scanning and electron microscopy work on nematodes. This research was supported by a grant from the United States-Israel Binational Agricultural Research and Development Fund (BARD; no. Us-1509-88) to T.R.M. and R.G. and a Charles and Johanna Research Award to T.R.M.",

year = "1993",

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doi = "10.1093/oxfordjournals.jhered.a111374",

language = "English (US)",

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PY - 1993/11

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N2 - Heterorhabditis bacteriophora is an entomopathogenic nematode that has potential as a biological control agent. As a first step in the genetic analysis of this nematode, we induced and isolated a short nematode (dumpy) from the F2progeny of an EMS mutagenized nematode. Phenotypically, the dumpy adult is 60% shorter than the wild type. There is almost no longitudinal growth after the infective juvenile stage. Dumpy is completely recessive with 100% penetrance and uniform expressivity. When F1progeny were allowed to self, F2progeny segregated wild: dumpy in a 15:1 ratio (χ2= 0.634,.5 >P >.2). This dumpy mutation (Rhdpy) complements another dumpy mutation isolated in Israel and is the second gene known for this species.

AB - Heterorhabditis bacteriophora is an entomopathogenic nematode that has potential as a biological control agent. As a first step in the genetic analysis of this nematode, we induced and isolated a short nematode (dumpy) from the F2progeny of an EMS mutagenized nematode. Phenotypically, the dumpy adult is 60% shorter than the wild type. There is almost no longitudinal growth after the infective juvenile stage. Dumpy is completely recessive with 100% penetrance and uniform expressivity. When F1progeny were allowed to self, F2progeny segregated wild: dumpy in a 15:1 ratio (χ2= 0.634,.5 >P >.2). This dumpy mutation (Rhdpy) complements another dumpy mutation isolated in Israel and is the second gene known for this species.

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Morphological mutant in the entomopathogenic nematode, heterorhabditis bacteriophora

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