@article{10fe6321eabc48d988f1800cdf55de91,
title = "Multifaceted characterization of cell wall decomposition products formed during ammonia fiber expansion (AFEX) and dilute acid based pretreatments",
abstract = "Decomposition products formed/released during ammonia fiber expansion (AFEX) and dilute acid (DA) pretreatment of corn stover (CS) were quantified using robust mass spectrometry based analytical platforms. Ammonolytic cleavage of cell wall ester linkages during AFEX resulted in the formation of acetamide (25. mg/g AFEX CS) and various phenolic amides (15. mg/g AFEX CS) that are effective nutrients for downstream fermentation. After ammonolysis, Maillard reactions with carbonyl-containing intermediates represent the second largest sink for ammonia during AFEX. On the other hand, several carboxylic acids were formed (e.g. 35. mg acetic acid/g DA CS) during DA pretreatment. Formation of furans was 36-fold lower for AFEX compared to DA treatment; while carboxylic acids (e.g. lactic and succinic acids) yield was 100-1000-fold lower during AFEX compared to previous reports using sodium hydroxide as pretreatment reagent.",
keywords = "AFEX, Ammonolysis, Degradation products, Dilute-acid pretreatment, Lignocellulosic corn stover",
author = "Chundawat, {Shishir P.S.} and Ramin Vismeh and Sharma, {Lekh N.} and Humpula, {James F.} and {da Costa Sousa}, Leonardo and Chambliss, {C. Kevin} and Jones, {A. Daniel} and Venkatesh Balan and Dale, {Bruce E.}",
note = "Funding Information: This work was funded by DOE Great Lakes Bioenergy Research Center ( www.greatlakesbioenergy.org ) supported by the U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, through Cooperative Agreement DE-FC02-07ER64494 between The Board of Regents of the University of Wisconsin System and the U. S. Department of Energy. We appreciate financial support, in initial stages of the project, from Michigan State Research Foundation (SPG grant), and CS/DATCS provided by NREL. The Quattro Premier mass spectrometer was purchased using funds from the NSF Major Research Instrumentation program (grant DBI-0619489 ). The participation of Baylor University researchers (LNS and CKC) was supported by the U.S. Department of Agriculture, National Institute of Food and Agriculture (formerly CSREES), National Research Initiative (Grants No. 2005-35504-16335 and 2008-35504-04612 ). We would like to thank Albert Cheh, John Ralph, Fachuang Lu, Per Askelano and other members of our laboratory for discussions and experimental assistance during the course of this project.",
year = "2010",
month = nov,
doi = "10.1016/j.biortech.2010.06.027",
language = "English (US)",
volume = "101",
pages = "8429--8438",
journal = "Bioresource Technology",
issn = "0960-8524",
publisher = "Elsevier Ltd",
number = "21",
}