Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array

Bernd Zetsche, Matthias Heidenreich, Prarthana Mohanraju, Iana Fedorova, Jeroen Kneppers, Ellen M. Degennaro, Nerges Winblad, Sourav R. Choudhury, Omar O. Abudayyeh, Jonathan S. Gootenberg, Wen Y. Wu, David A. Scott, Konstantin Severinov, John Van Der Oost, Feng Zhang

Research output: Contribution to journalReview articlepeer-review

410 Scopus citations

Abstract

Targeting of multiple genomic loci with Cas9 is limited by the need for multiple or large expression constructs. Here we show that the ability of Cpf1 to process its own CRISPR RNA (crRNA) can be used to simplify multiplexed genome editing. Using a single customized CRISPR array, we edit up to four genes in mammalian cells and three in the mouse brain, simultaneously.

Original languageEnglish (US)
Pages (from-to)31-34
Number of pages4
JournalNature biotechnology
Volume35
Issue number1
DOIs
StatePublished - Jan 1 2017

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Fingerprint

Dive into the research topics of 'Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array'. Together they form a unique fingerprint.

Cite this