Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains

Liang Chen, Kalyan D. Chavda, Jacqueline Findlay, Gisele Peirano, Katie Hopkins, Johann D.D. Pitout, Robert A. Bonomo, Neil Woodford, Frank R. DeLeo, Barry N. Kreiswirth

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

We developed a multiplex PCR assay capable of identifying two capsular polysaccharide synthesis sequence types (sequence type 258 [ST258] cps-1 and cps-2) in epidemic Klebsiella pneumoniae ST258 strains. The assay performed with excellent sensitivity (100%) and specificity (100%) for identifying cps types in 60 ST258 K. pneumoniae sequenced isolates. The screening of 419 ST258 clonal isolates revealed a significant association between cps type and K. pneumoniae carbapenemase (KPC) variant: cps-1 is largely associated with KPC-2, while cps-2 is primarily associated with KPC-3.

Original languageEnglish (US)
Pages (from-to)4196-4199
Number of pages4
JournalAntimicrobial agents and chemotherapy
Volume58
Issue number7
DOIs
StatePublished - Jul 2014

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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