Mutants of Saccharomyces cerevisiae deficient in adenine phosphoribosyltransferase

Amrik Sahota, Prabhakar K. Ranjekar, Juan Alfonzo, Alfred S. Lewin, Milton W. Taylor

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Spontaneous and ethyl methanesulfate induced mutants of Saccharomyces cerevisiae, with partial and complete deficiency of adenine phosphoribosyltransferase (APRT, EC 2.4.2.7), were isolated by selection for resistance to 8-azaadenine. Matings between totally deficient mutants and tester strain resulted in diploid heterozygotes that were sensitive to azaadenine. Upon sporulation and tetrad analysis, azaadenine resistance (and APRT deficiency) segregated as expected for a single Mendelian gene. Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) activity in the mutants was similar to that in the wild-type cells. There was no detectable activity of adenine aminohydrolase (EC 3.5.4.2) in the wild-type or mutant cells.

Original languageEnglish (US)
Pages (from-to)81-87
Number of pages7
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume180
Issue number1
DOIs
StatePublished - Sep 1987
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

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