Mutational analysis of SEC4 suggests a cyclical mechanism for the regulation of vesicular traffic

N. C. Walworth, B. Goud, A. Kastan Kabcenell, P. J. Novick

Research output: Contribution to journalArticle

190 Scopus citations

Abstract

Mutant alleles of SEC4, an essential gene required for the final stage of secretion in yeast, have been generated by in vitro mutagenesis. Deletion of the two cystein residues at the C terminus of the protein results in a soluble non-functional protein, indicating that those two residues are required for normal localization of Sec4p to secretory vesicles and the plasma membrane. A mutant allele of SEC4 generated to mimic an activated, transforming allele of H-ras, as predicted, does not bind GTP. The presence of this allele in cells containing wild-type SEC4 causes a secretory defect and the accumulation of secretory vesicles. The results of genetic studies indicate that this allele behaves as a dominant loss of function mutant and as such prevents wild-type protein from functioning properly. We propose a model in which Sec4p cycles between an active and an inactive state in order to mediate the fusion of vesicles to the plasma membrane.

Original languageEnglish (US)
Pages (from-to)1685-1693
Number of pages9
JournalEMBO Journal
Volume8
Issue number6
DOIs
StatePublished - Jan 1 1989

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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