Mutations upstream of the ribosome-binding site affect translational efficiency

Jack Coleman, Masayori Inouye, Kenzo Nakamura

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


The DNA coding for the major outer membrane lipoprotein of Escherichia coli has been fused to the coding region of the β-galactosidase gene to measure the effect of various mutations on the efficiency of translation initiation. The various mutants were made by either inserting or deleting a small number of nucleotides into or from a region just upstream of the ribosome-binding site. These small mutations dramatically affect translation initiation as measured by the production of β-galactosidase. We postulate that these mutations affect translation initiation by altering the secondary structure of the messenger RNA. In one case, we predict that a stem and loop just upstream of the Shine-Dalgarno sequence sterically hinders the binding of the ribosome to the mRNA.

Original languageEnglish (US)
Pages (from-to)139-143
Number of pages5
JournalJournal of molecular biology
Issue number1
StatePublished - Jan 5 1985
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology


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