The DNA coding for the major outer membrane lipoprotein of Escherichia coli has been fused to the coding region of the β-galactosidase gene to measure the effect of various mutations on the efficiency of translation initiation. The various mutants were made by either inserting or deleting a small number of nucleotides into or from a region just upstream of the ribosome-binding site. These small mutations dramatically affect translation initiation as measured by the production of β-galactosidase. We postulate that these mutations affect translation initiation by altering the secondary structure of the messenger RNA. In one case, we predict that a stem and loop just upstream of the Shine-Dalgarno sequence sterically hinders the binding of the ribosome to the mRNA.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of molecular biology|
|State||Published - Jan 5 1985|
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology