TY - JOUR
T1 - Nalidixic acid-sensitive and resistant modes of DNA replication in Escherichia coli
AU - Dermody, James J.
AU - Bourguignon, Gerard J.
AU - Foglesong, P. David
AU - Sternglanz, Rolf
N1 - Funding Information:
was supported by grants from the American Cancer Society (NP-142) and the
PY - 1974/12/23
Y1 - 1974/12/23
N2 - The bacterial DNA synthesis inhibitor, nalidixic acid, inhibits DNA replication to different extents in various strains of E. coli, and the amount of inhibition is dependent on the growth temperature. DNA synthesis in the temperature-sensitive DNA replication mutant, dna F (a mutant with a defective ribonucleotide reductase), is sensitive to nalidixic acid at the permissive temperature, but is unusually resistant to nalidixic acid at the non-permissive temperature. This behavior is directly correlated with the dna F mutation, since it is not present in the parent strain of dna F, nor in a temperature-independent revertant of dna F. The results are discussed in terms of two modes of DNA replication, one sensitive to nalidixic acid and the other resistant to the drug.
AB - The bacterial DNA synthesis inhibitor, nalidixic acid, inhibits DNA replication to different extents in various strains of E. coli, and the amount of inhibition is dependent on the growth temperature. DNA synthesis in the temperature-sensitive DNA replication mutant, dna F (a mutant with a defective ribonucleotide reductase), is sensitive to nalidixic acid at the permissive temperature, but is unusually resistant to nalidixic acid at the non-permissive temperature. This behavior is directly correlated with the dna F mutation, since it is not present in the parent strain of dna F, nor in a temperature-independent revertant of dna F. The results are discussed in terms of two modes of DNA replication, one sensitive to nalidixic acid and the other resistant to the drug.
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U2 - 10.1016/S0006-291X(74)80431-6
DO - 10.1016/S0006-291X(74)80431-6
M3 - Article
C2 - 4616700
AN - SCOPUS:0016326863
VL - 61
SP - 1340
EP - 1347
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 4
ER -