Nanomechanics of knockout mouse bones

N. Beril Kavukcuoglu, Adrian Mann

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Osteocalcin (OC) and osteopontin (OPN) are among the most abundant non-collagenous bone matrix proteins. Both have drawn interest from investigators studying their function in osteoporosis, and it is known that mutations of these proteins can also have dramatic effects on the properties of bone. Other proteins including fibrillin 1 and 2 (FBN2) have been less widely studied, but can be mutated in some individuals resulting in connective tissue disorders. It has been reported that abnormal fibrillin may play a role in decreased bone mass. In this study, bones from osteopontin (OPN), osteocalcin (OC) and fibrillin-2 (FBN2) knockout mice have been investigated. The study has identified how these proteins affect the bone's nanomechanical properties (hardness and elastic modulus). Nanoindentation tests were performed on the radial axis of cortical femora bones from the knockout mice and their wildtype controls. The results showed that young (age< 12 weeks) OPN knock-out bones have significantly lower mechanical properties than wild-type bones, indicating a crucial role for OPN in early bone mineralization. After 12 weeks of age, the OPN knockout and wild-type control bones did not show any statistical difference. In OC deficient mice the mechanical properties were found to increase in the cortical mid-shaft of femora from 1 year old mice, suggesting an increase in bone mineralization, but 3 month old FBN2 deficient mice bones showed a decrease in mechanical properties across the cortical radial axis of the mid- femora.

Original languageEnglish (US)
Title of host publicationMechanics of Biological and Bio-Inspired Materials
Pages141-146
Number of pages6
StatePublished - Dec 1 2006
Event2006 MRS Fall Meeting - Boston, MA, United States
Duration: Nov 27 2006Dec 1 2006

Publication series

NameMaterials Research Society Symposium Proceedings
Volume975
ISSN (Print)0272-9172

Other

Other2006 MRS Fall Meeting
CountryUnited States
CityBoston, MA
Period11/27/0612/1/06

Fingerprint

Nanomechanics
Bone
Osteopontin
Osteocalcin
Proteins
Mechanical properties
Nanoindentation

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Kavukcuoglu, N. B., & Mann, A. (2006). Nanomechanics of knockout mouse bones. In Mechanics of Biological and Bio-Inspired Materials (pp. 141-146). (Materials Research Society Symposium Proceedings; Vol. 975).
Kavukcuoglu, N. Beril ; Mann, Adrian. / Nanomechanics of knockout mouse bones. Mechanics of Biological and Bio-Inspired Materials. 2006. pp. 141-146 (Materials Research Society Symposium Proceedings).
@inproceedings{a0ce1f5095ba4d4e81d00e5f7f4db42d,
title = "Nanomechanics of knockout mouse bones",
abstract = "Osteocalcin (OC) and osteopontin (OPN) are among the most abundant non-collagenous bone matrix proteins. Both have drawn interest from investigators studying their function in osteoporosis, and it is known that mutations of these proteins can also have dramatic effects on the properties of bone. Other proteins including fibrillin 1 and 2 (FBN2) have been less widely studied, but can be mutated in some individuals resulting in connective tissue disorders. It has been reported that abnormal fibrillin may play a role in decreased bone mass. In this study, bones from osteopontin (OPN), osteocalcin (OC) and fibrillin-2 (FBN2) knockout mice have been investigated. The study has identified how these proteins affect the bone's nanomechanical properties (hardness and elastic modulus). Nanoindentation tests were performed on the radial axis of cortical femora bones from the knockout mice and their wildtype controls. The results showed that young (age< 12 weeks) OPN knock-out bones have significantly lower mechanical properties than wild-type bones, indicating a crucial role for OPN in early bone mineralization. After 12 weeks of age, the OPN knockout and wild-type control bones did not show any statistical difference. In OC deficient mice the mechanical properties were found to increase in the cortical mid-shaft of femora from 1 year old mice, suggesting an increase in bone mineralization, but 3 month old FBN2 deficient mice bones showed a decrease in mechanical properties across the cortical radial axis of the mid- femora.",
author = "Kavukcuoglu, {N. Beril} and Adrian Mann",
year = "2006",
month = "12",
day = "1",
language = "English (US)",
isbn = "9781604234237",
series = "Materials Research Society Symposium Proceedings",
pages = "141--146",
booktitle = "Mechanics of Biological and Bio-Inspired Materials",

}

Kavukcuoglu, NB & Mann, A 2006, Nanomechanics of knockout mouse bones. in Mechanics of Biological and Bio-Inspired Materials. Materials Research Society Symposium Proceedings, vol. 975, pp. 141-146, 2006 MRS Fall Meeting, Boston, MA, United States, 11/27/06.

Nanomechanics of knockout mouse bones. / Kavukcuoglu, N. Beril; Mann, Adrian.

Mechanics of Biological and Bio-Inspired Materials. 2006. p. 141-146 (Materials Research Society Symposium Proceedings; Vol. 975).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Nanomechanics of knockout mouse bones

AU - Kavukcuoglu, N. Beril

AU - Mann, Adrian

PY - 2006/12/1

Y1 - 2006/12/1

N2 - Osteocalcin (OC) and osteopontin (OPN) are among the most abundant non-collagenous bone matrix proteins. Both have drawn interest from investigators studying their function in osteoporosis, and it is known that mutations of these proteins can also have dramatic effects on the properties of bone. Other proteins including fibrillin 1 and 2 (FBN2) have been less widely studied, but can be mutated in some individuals resulting in connective tissue disorders. It has been reported that abnormal fibrillin may play a role in decreased bone mass. In this study, bones from osteopontin (OPN), osteocalcin (OC) and fibrillin-2 (FBN2) knockout mice have been investigated. The study has identified how these proteins affect the bone's nanomechanical properties (hardness and elastic modulus). Nanoindentation tests were performed on the radial axis of cortical femora bones from the knockout mice and their wildtype controls. The results showed that young (age< 12 weeks) OPN knock-out bones have significantly lower mechanical properties than wild-type bones, indicating a crucial role for OPN in early bone mineralization. After 12 weeks of age, the OPN knockout and wild-type control bones did not show any statistical difference. In OC deficient mice the mechanical properties were found to increase in the cortical mid-shaft of femora from 1 year old mice, suggesting an increase in bone mineralization, but 3 month old FBN2 deficient mice bones showed a decrease in mechanical properties across the cortical radial axis of the mid- femora.

AB - Osteocalcin (OC) and osteopontin (OPN) are among the most abundant non-collagenous bone matrix proteins. Both have drawn interest from investigators studying their function in osteoporosis, and it is known that mutations of these proteins can also have dramatic effects on the properties of bone. Other proteins including fibrillin 1 and 2 (FBN2) have been less widely studied, but can be mutated in some individuals resulting in connective tissue disorders. It has been reported that abnormal fibrillin may play a role in decreased bone mass. In this study, bones from osteopontin (OPN), osteocalcin (OC) and fibrillin-2 (FBN2) knockout mice have been investigated. The study has identified how these proteins affect the bone's nanomechanical properties (hardness and elastic modulus). Nanoindentation tests were performed on the radial axis of cortical femora bones from the knockout mice and their wildtype controls. The results showed that young (age< 12 weeks) OPN knock-out bones have significantly lower mechanical properties than wild-type bones, indicating a crucial role for OPN in early bone mineralization. After 12 weeks of age, the OPN knockout and wild-type control bones did not show any statistical difference. In OC deficient mice the mechanical properties were found to increase in the cortical mid-shaft of femora from 1 year old mice, suggesting an increase in bone mineralization, but 3 month old FBN2 deficient mice bones showed a decrease in mechanical properties across the cortical radial axis of the mid- femora.

UR - http://www.scopus.com/inward/record.url?scp=41549157219&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41549157219&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:41549157219

SN - 9781604234237

T3 - Materials Research Society Symposium Proceedings

SP - 141

EP - 146

BT - Mechanics of Biological and Bio-Inspired Materials

ER -

Kavukcuoglu NB, Mann A. Nanomechanics of knockout mouse bones. In Mechanics of Biological and Bio-Inspired Materials. 2006. p. 141-146. (Materials Research Society Symposium Proceedings).