Nanopatterned hydrogel probes for rapid detection of staphylococcus aureus

Ishtiaq Saaem, Barry Kreiswirth, Matthew Libera

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We studied the use of nanopatterned hydrogels as a possible foundation for medical devices for the detection of Staphylococcus aureus. Nanopatterned hydrogels, approximately 200 nm in diameter, were created by locally crosslinking dry amine-terminated poly(ethylene glycol) [PEG] (6000 Da) thin films using a focused electron beam. These gels then had a dry height of 50 nm and a swell ratio of about five. They were patterned into arrays with approximately 500 nm inter-gel spacing. These arrayed gels were functionalized with Immunoglobulin G [IgG], a biomolecule that binds protein A. We are interested in seeing whether these arrayed gels can be used for bacterial capture and subsequent detection. We show, using an assay that binds IgG to protein A expressing Staphylococcus aureus [S. aureus], that nanopatterned hydrogels can indeed be used as probes for detection. We further show that the specificity of our assay by using a knock-out variant of S. aureus that doesn't express protein A as a negative control.

Original languageEnglish (US)
Title of host publicationBiofilm-Material Interactions
Subtitle of host publicationNew Tools, Technologies and Opportunities
PublisherMaterials Research Society
Pages14-19
Number of pages6
ISBN (Print)9781604234107
DOIs
StatePublished - 2006
Externally publishedYes
Event2006 MRS Fall Meeting - Boston, MA, United States
Duration: Nov 27 2006Dec 1 2006

Publication series

NameMaterials Research Society Symposium Proceedings
Volume954
ISSN (Print)0272-9172

Other

Other2006 MRS Fall Meeting
Country/TerritoryUnited States
CityBoston, MA
Period11/27/0612/1/06

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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