This paper presents a flexible approach for using Dip Pen Nanolithography (DPN) to nanopattern mixed monolayers for the selective immobilization of bioassemblies. DPN was used with a binary ink - consisting of a symmetric 11-mercaptoundecyl-penta(ethylene glycol) disulfide and a mixed disulfide substituted with one maleimide group - to pattern nanoscale features that present functional groups for the chemospecific immobilization of cysteine-labeled biomolecules. This strategy was applied to the chemospecific immobilization of cysteine mutant cowpea mosaic virus capsid particles (cys-VCPs). The combination of DPN for defining nanopatterns and surface chemistries for controlling the immobilization of ligands will be broadly useful in basic and applied biology.
All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Condensed Matter Physics
- Mechanical Engineering