Nanopatterning the chemospecific immobilization of cowpea mosaic virus capsid

Jennifer C. Smith, Ki Bum Lee, Qian Wang, M. G. Finn, John E. Johnson, Milan Mrksich, Chad A. Mirkin

Research output: Contribution to journalArticle

138 Scopus citations


This paper presents a flexible approach for using Dip Pen Nanolithography (DPN) to nanopattern mixed monolayers for the selective immobilization of bioassemblies. DPN was used with a binary ink - consisting of a symmetric 11-mercaptoundecyl-penta(ethylene glycol) disulfide and a mixed disulfide substituted with one maleimide group - to pattern nanoscale features that present functional groups for the chemospecific immobilization of cysteine-labeled biomolecules. This strategy was applied to the chemospecific immobilization of cysteine mutant cowpea mosaic virus capsid particles (cys-VCPs). The combination of DPN for defining nanopatterns and surface chemistries for controlling the immobilization of ligands will be broadly useful in basic and applied biology.

Original languageEnglish (US)
Pages (from-to)883-886
Number of pages4
JournalNano Letters
Issue number7
StatePublished - Jul 1 2003
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Smith, J. C., Lee, K. B., Wang, Q., Finn, M. G., Johnson, J. E., Mrksich, M., & Mirkin, C. A. (2003). Nanopatterning the chemospecific immobilization of cowpea mosaic virus capsid. Nano Letters, 3(7), 883-886.