NCAM promotes assembly and activity-dependent remodeling of the postsynaptic signaling complex

Vladimir Sytnyk, Iryna Leshchyns'Ka, Alexander G. Nikonenko, Melitta Schachner

Research output: Contribution to journalArticlepeer-review

92 Scopus citations


The neural cell adhesion molecule (NCAM) regulates synapse formation and synaptic strength via mechanisms that have remained unknown. We show that NCAM associates with the postsynaptic spectrin-based scaffold, cross-linking NCAM with the N-methyl-D-aspartate (NMDA) receptor and Ca2+/calmodulin- dependent protein kinase II α (CaMKIIα) in a manner not firmly or directly linked to PSD95 and α-actinin. Clustering of NCAM promotes formation of detergent-insoluble complexes enriched in postsynaptic proteins and resembling postsynaptic densities. Disruption of the NCAM-spectrin complex decreases the size of postsynaptic densities and reduces synaptic targeting of NCAM-spectrin-associated postsynaptic proteins, including spectrin, NMDA receptors, and CaMKIIα. Degeneration of the spectrin scaffold in NCAM-deficient neurons results in an inability to recruit CaMKIIα to synapses after NMDA receptor activation, which is a critical process in NMDA receptor-dependent long-term potentiation. The combined observations indicate that NCAM promotes assembly of the spectrin-based postsynaptic signaling complex, which is required for activity-associated, long-lasting changes in synaptic strength. Its abnormal function may contribute to the etiology of neuropsychiatric disorders associated with mutations in or abnormal expression of NCAM.

Original languageEnglish (US)
Pages (from-to)1071-1085
Number of pages15
JournalJournal of Cell Biology
Issue number7
StatePublished - Sep 25 2006

All Science Journal Classification (ASJC) codes

  • Cell Biology

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