NDF is a transcription factor that stimulates elongation by RNA polymerase II

Jia Fei; Jun Xu; Ziwei Li; Kevin Xu; Dong Wang; George A. Kassavetis; James T. Kadonaga

doi:10.1101/gad.349150.121

NDF is a transcription factor that stimulates elongation by RNA polymerase II

Jia Fei, Jun Xu, Ziwei Li, Kevin Xu, Dong Wang, George A. Kassavetis, James T. Kadonaga

School of Arts and Sciences, Molecular Biology & Biochemistry

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

RNA polymerase II (Pol II) elongation is a critical step in gene expression. Here we found that NDF, which was identified as a bilaterian nucleosome-destabilizing factor, is also a Pol II transcription factor that stimulates elongation with plain DNA templates in the absence of nucleosomes. NDF binds directly to Pol II and enhances elongation by a different mechanism than that used by transcription factor TFIIS. Moreover, yeast Pdp3, which is related to NDF, binds to Pol II and stimulates elongation. Thus, NDF is a Pol II binding transcription elongation factor that is localized over gene bodies and is conserved from yeast to humans.

Original language	English (US)
Pages (from-to)	294-299
Number of pages	6
Journal	Genes and Development
Volume	34
Issue number	5-6
DOIs	https://doi.org/10.1101/gad.349150.121
State	Published - Mar 1 2022

All Science Journal Classification (ASJC) codes

General Medicine

Keywords

NDF
RNA polymerase II
TFIIS
elongation
gene expression]
transcription

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10.1101/gad.349150.121

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title = "NDF is a transcription factor that stimulates elongation by RNA polymerase II",

abstract = "RNA polymerase II (Pol II) elongation is a critical step in gene expression. Here we found that NDF, which was identified as a bilaterian nucleosome-destabilizing factor, is also a Pol II transcription factor that stimulates elongation with plain DNA templates in the absence of nucleosomes. NDF binds directly to Pol II and enhances elongation by a different mechanism than that used by transcription factor TFIIS. Moreover, yeast Pdp3, which is related to NDF, binds to Pol II and stimulates elongation. Thus, NDF is a Pol II binding transcription elongation factor that is localized over gene bodies and is conserved from yeast to humans.",

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AU - Fei, Jia

AU - Xu, Jun

AU - Li, Ziwei

AU - Xu, Kevin

AU - Wang, Dong

AU - Kassavetis, George A.

AU - Kadonaga, James T.

PY - 2022/3/1

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AB - RNA polymerase II (Pol II) elongation is a critical step in gene expression. Here we found that NDF, which was identified as a bilaterian nucleosome-destabilizing factor, is also a Pol II transcription factor that stimulates elongation with plain DNA templates in the absence of nucleosomes. NDF binds directly to Pol II and enhances elongation by a different mechanism than that used by transcription factor TFIIS. Moreover, yeast Pdp3, which is related to NDF, binds to Pol II and stimulates elongation. Thus, NDF is a Pol II binding transcription elongation factor that is localized over gene bodies and is conserved from yeast to humans.

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NDF is a transcription factor that stimulates elongation by RNA polymerase II

Abstract

All Science Journal Classification (ASJC) codes

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