Networks of gold nanoparticles and bacteriophage as biological sensors and cell-targetting agents

Glauco R. Souza, Dawn R. Christianson, Fernanda I. Staquicini, Michael G. Ozawa, Evan Y. Snyder, Richard L. Sidman, J. Houston Miller, Wadih Arap, Renata Pasqualini

Research output: Contribution to journalArticlepeer-review

233 Scopus citations


Biological molecular assemblies are excellent models for the development of nanoengineered systems with desirable biomedical properties. Here we report an approach for fabrication of spontaneous, biologically active molecular networks consisting of bacteriophage (phage) directly assembled with gold (Au) nanoparticles (termed Au-phage). We show that when the phage are engineered so that each phage particle displays a peptide, such networks preserve the cell surface receptor binding and internalization attributes of the displayed peptide. The spontaneous organization of these targeted networks can be manipulated further by incorporation of imidazole (Au-phage-imid), which induces changes in fractal structure and near-infrared optical properties. The networks can be used as labels for enhanced fluorescence and dark-field microscopy, surface-enhanced Raman scattering detection, and near-infrared photon-to-heat conversion. Together, the physical and biological features within these targeted networks offer convenient multifunctional integration within a single entity with potential for nanotechnology-based biomedical applications.

Original languageEnglish (US)
Pages (from-to)1215-1220
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number5
StatePublished - Jan 31 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General


  • Assembly
  • Fractal
  • Hydrogel
  • Stem cell
  • Target


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