Transcription in the human and rat D(1A) dopamine receptor genes proceeds from two distinct promoters in neuronal cells while only the downstream intronic promoter is active in renal cells. To investigate the utility of these promoters in the brain cell-specific expression of transgenes, we now studied the 5' flanking region of the murine D(1A) gene. We confirmed the presence of two functional promoters utilized for the tissue-specific regulation of this gene similar to its human and rat homologues. The cloned 1.4-kb genomic fragment spans nucleotides -967 to +384 relative to the first ATG codon and includes intron 1 between bases -534 to -420. Transient expression analyses using various chroramphenicol acetyltransferase constructs revealed that the murine D(1A) upstream promoter fused with the human D(1A) gene activator sequence ActAR1 has potent transcriptional activity in a D(1A)-expressing neuronal cell line but not in other cell lines tested including renal (OK cells), glial (C6) and hepatic (HepG2), suggesting that this hybrid construct harbors neural cell-specific elements. The availability of potent regulatory DNA cassettes harboring the murine D(1A) gene promoter could aid testing the neuronal-specific expression of transgenes in vivo. Copyright (C) 1999 Elsevier Science Ireland Ltd.
|Original language||English (US)|
|Number of pages||10|
|State||Published - Sep 1999|
All Science Journal Classification (ASJC) codes
- Chloramphenicol acetyl transferase
- DNA sequence