Neurite Extension by Mouse Neuroblastoma: Evidence for Two Modes of Induction

The induction of neurite extension by mouse neuroblastoma cells in vitro can be reversed by adding colchicine or cytochalasin B (CB) to the culture medium. The relative sensitivity of the neurites to retraction is a function of the method used to induce extension. Cells incubated in the absence of serum or in the presence of serum and dibutyryl cyclic-AMP are sensitive to both colchicine and CB; cells incubated in serum-free medium containing either cycloheximide or dimethylsulphoxide are sensitive only to colchicine.